INSTRUMENTATION FOR SPECTRAL KARYOTYPING

光谱核型分析是一种发展中的细胞遗传学技术，用于识别在癌细胞系中出现的染色体重排，如缺失、倒置和易位。一种商业仪器的操作方式是安装在外照显微镜上的改进型萨格纳克干涉仪，以捕捉用于绘制染色体的探针的光谱特征。显微镜的表观照明端口和荧光立方体组件已被修改为允许单波长激发，导致荧光信号的信噪比提高了一个数量级以上，并降低了整个背景信号。这些改进将允许解决较小的染色体条带异常，并确定它们与疾病的相关性。-天空、光谱核型、细胞遗传学、染色体异常、仪器

Spectral karyotyping is an evolving cytogenetic technology to identify chromosomal rearrangements such as deletions, inversions, and translocations seen in cancer cell lines. A commercial instrument operates as a modified Sagnac interferometer installed on an epi- illumination microscope to capture the spectral signature of probes used to paint chromosomes. The epi-illumination port and the fluorescent cube assembly of the microscope have been modified to permit single wavelength excitation, resulting in greater than an order of magnitude improvement in the signal to noise ratio of the fluorescent signals and a reduction in the overall background signal. These improvements will permit resolution of smaller chromosome band abnormalities and identification of their correlation with disease. - SKY, spectral karyotyping, cytogenetics, chromosomal abnormality, instrumentation