INSTRUMENTATION FOR SPECTRAL KARYOTYPING

光谱核型是一种不断发展的细胞遗传学技术，用于鉴定癌细胞系中出现的染色体重排，如缺失、倒位和易位。一种商用仪器作为一种改进的Sagnac干涉仪安装在外照显微镜上，以捕获用于染色的探针的光谱特征。显微镜的延伸照明端口和荧光立方体组件已被修改，以允许单波长激发，导致在荧光信号的信噪比大于一个数量级的改善，并在整体背景信号的减少。这些改进将允许解决较小的染色体带异常，并确定其与疾病的相关性。- SKY，光谱核型，细胞遗传学，染色体异常，仪器

Spectral karyotyping is an evolving cytogenetic technology to identify chromosomal rearrangements such as deletions, inversions, and translocations seen in cancer cell lines. A commercial instrument operates as a modified Sagnac interferometer installed on an epi- illumination microscope to capture the spectral signature of probes used to paint chromosomes. The epi-illumination port and the fluorescent cube assembly of the microscope have been modified to permit single wavelength excitation, resulting in greater than an order of magnitude improvement in the signal to noise ratio of the fluorescent signals and a reduction in the overall background signal. These improvements will permit resolution of smaller chromosome band abnormalities and identification of their correlation with disease. - SKY, spectral karyotyping, cytogenetics, chromosomal abnormality, instrumentation