BLUE LIGHT EMITTING DIODE FOR FLUORESCENCE SENSING

用于荧光传感的蓝光发光二极管

• 批准号: 6444720
• 负责人: GOVIND RAO
• 金额: $ 29.31万
• 依托单位: UNIVERSITY OF MARYLAND BALTIMORE
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-04-01 至 2002-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6444720
• 关键词: biological products; biomaterials; biomedical equipment development; biomedical resource; biotechnology; spectrometry

We have produced amplitude-modulated near-ultraviolet light, centered at 390 nm, using an inexpensive, commercially available blue light emitting diode (LED). The fluorescence lifetimes of standard fluorophores were measured in the frequency -domain using the phase modulation technique, producing fluorescence lifetimes that agree with those reported in the literature. The ability to modulate the LED at high frequencies, along with the UV output, allows its use as an inexpensive UV light source in the fluorescence lifetime optical sensors and even frequency-domain fluorometers.

我们产生了调幅近紫外光， 以 390 nm 为中心，使用廉价的市售蓝色 发光二极管（LED）。 标准荧光寿命 使用相位在频域中测量荧光团 调制技术，产生符合的荧光寿命 文献中报道的那些。 调制 LED 的能力 高频以及紫外线输出使其可以用作 荧光寿命光学中廉价的紫外光源 传感器甚至频域荧光计。