MECHANISM AND FUNCTION OF UNIPOLARITY OF SHIGELLA ICSA
志贺氏菌ICSA单极性的机制和作用
基本信息
- 批准号:6475699
- 负责人:
- 金额:$ 34.01万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1994
- 资助国家:美国
- 起止时间:1994-08-01 至 2002-01-02
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
DESCRIPTION (Adapted from the Applicant's Abstract): Shigella spp. continue to
be a leading cause of dysentery and diarrhea annually worldwide. Shigella is
unique among Gram-negative enteric pathogens in that it accesses the cytoplasm
of host cells and assembles actin into long tails, which propel bacterial
spread through tissues. The investigators have previously shown that the
Shigella outer membrane protein IcsA is sufficient for actin assembly and that
IcsA is localized to a single pole of the bacillus. The molecular mechanisms
involved in the unipolar localization of IcsA are unknown. Their data
demonstrate that IcsA is directly targeted to the bacterial pole. In
conjunction with this, they have developed constructs that provide the tools
necessary to directly address the molecular mechanisms of unipolar targeting of
IcsA, a major goal of this proposal. In contrast, IcsA that is uniformly
distributed over the surface of certain E. coli strains is able to mediate
actin tail assembly without capping of the IcsA. This proposal also
specifically addresses the function of unipolar localization of IcsA in
Shigella pathogenesis, which they are now in a position to test critically.
The Specific Aims of this proposal are: (1) the identification and
characterization of residues and domains of IcsA required for its unipolar
localization; (2) the identification of Shigella proteins that directly
interact with IcsA and analysis of their potential role in IcsA unipolar
localization; and (3) an assessment of the role of IcsA unipolarity in Shigella
pathogenesis. This application proposes to obtain information that will provide
insight into the molecular mechanisms of unipolar targeting of the S. flexneri
virulence factor IcsA. Further, their studies will elucidate the role of IcsA
unipolarity in the pathogenesis and virulence of Shigella. Finally, their
studies will likely provide insight into the fundamental mechanisms that
mediate three-dimensional targeting of proteins in bacteria and the molecular
characteristics of the bacterial old pole that distinguish it from the new pole
and the sides of the bacillus.
描述(改编自申请人摘要):志贺氏菌属
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Marcia B Goldberg其他文献
Marcia B Goldberg的其他文献
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{{ truncateString('Marcia B Goldberg', 18)}}的其他基金
Human NLRP11 function in non-canonical inflammasome activation by bacterial pathogen LPS
人类NLRP11在细菌病原体LPS非典型炎症小体激活中的作用
- 批准号:
10563477 - 财政年份:2023
- 资助金额:
$ 34.01万 - 项目类别:
Infectious Disease and Basic Microbiological Mechanisms
传染病和基本微生物机制
- 批准号:
9411265 - 财政年份:2016
- 资助金额:
$ 34.01万 - 项目类别:
Bacterial cell envelope in polar positioning of autotransporter proteins
自转运蛋白极性定位中的细菌细胞包膜
- 批准号:
8917850 - 财政年份:2014
- 资助金额:
$ 34.01万 - 项目类别:
Bacterial cell envelope in polar positioning of autotransporter proteins
自转运蛋白极性定位中的细菌细胞包膜
- 批准号:
8638264 - 财政年份:2014
- 资助金额:
$ 34.01万 - 项目类别:
Shigella repression of innate immunity early during infection
志贺氏菌在感染早期抑制先天免疫
- 批准号:
8853815 - 财政年份:2014
- 资助金额:
$ 34.01万 - 项目类别:
Shigella repression of innate immunity early during infection
志贺氏菌在感染早期抑制先天免疫
- 批准号:
8772174 - 财政年份:2014
- 资助金额:
$ 34.01万 - 项目类别:
The cellular filopodia mechanism in Shigella membrane protrusion formation
志贺氏菌膜突起形成的细胞丝状伪足机制
- 批准号:
8607891 - 财政年份:2013
- 资助金额:
$ 34.01万 - 项目类别:
The cellular filopodia mechanism in Shigella membrane protrusion formation
志贺氏菌膜突起形成的细胞丝状伪足机制
- 批准号:
8430385 - 财政年份:2013
- 资助金额:
$ 34.01万 - 项目类别:
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Priority Programmes
STRUCTURE/INTERACTIONS OF ACTINS AND ACTIN-BINDING PROTEIN
肌动蛋白和肌动蛋白结合蛋白的结构/相互作用
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