MECHANISM AND FUNCTION OF UNIPOLARITY OF SHIGELLA ICSA

志贺氏菌ICSA单极性的机制和作用

基本信息

  • 批准号:
    6475699
  • 负责人:
  • 金额:
    $ 34.01万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1994
  • 资助国家:
    美国
  • 起止时间:
    1994-08-01 至 2002-01-02
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (Adapted from the Applicant's Abstract): Shigella spp. continue to be a leading cause of dysentery and diarrhea annually worldwide. Shigella is unique among Gram-negative enteric pathogens in that it accesses the cytoplasm of host cells and assembles actin into long tails, which propel bacterial spread through tissues. The investigators have previously shown that the Shigella outer membrane protein IcsA is sufficient for actin assembly and that IcsA is localized to a single pole of the bacillus. The molecular mechanisms involved in the unipolar localization of IcsA are unknown. Their data demonstrate that IcsA is directly targeted to the bacterial pole. In conjunction with this, they have developed constructs that provide the tools necessary to directly address the molecular mechanisms of unipolar targeting of IcsA, a major goal of this proposal. In contrast, IcsA that is uniformly distributed over the surface of certain E. coli strains is able to mediate actin tail assembly without capping of the IcsA. This proposal also specifically addresses the function of unipolar localization of IcsA in Shigella pathogenesis, which they are now in a position to test critically. The Specific Aims of this proposal are: (1) the identification and characterization of residues and domains of IcsA required for its unipolar localization; (2) the identification of Shigella proteins that directly interact with IcsA and analysis of their potential role in IcsA unipolar localization; and (3) an assessment of the role of IcsA unipolarity in Shigella pathogenesis. This application proposes to obtain information that will provide insight into the molecular mechanisms of unipolar targeting of the S. flexneri virulence factor IcsA. Further, their studies will elucidate the role of IcsA unipolarity in the pathogenesis and virulence of Shigella. Finally, their studies will likely provide insight into the fundamental mechanisms that mediate three-dimensional targeting of proteins in bacteria and the molecular characteristics of the bacterial old pole that distinguish it from the new pole and the sides of the bacillus.
描述(改编自申请人摘要):志贺氏菌属

项目成果

期刊论文数量(0)
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科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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Marcia B Goldberg其他文献

Marcia B Goldberg的其他文献

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{{ truncateString('Marcia B Goldberg', 18)}}的其他基金

Human NLRP11 function in non-canonical inflammasome activation by bacterial pathogen LPS
人类NLRP11在细菌病原体LPS非典型炎症小体激活中的作用
  • 批准号:
    10563477
  • 财政年份:
    2023
  • 资助金额:
    $ 34.01万
  • 项目类别:
Infectious Disease and Basic Microbiological Mechanisms
传染病和基本微生物机制
  • 批准号:
    9411265
  • 财政年份:
    2016
  • 资助金额:
    $ 34.01万
  • 项目类别:
Bacterial cell envelope in polar positioning of autotransporter proteins
自转运蛋白极性定位中的细菌细胞包膜
  • 批准号:
    8917850
  • 财政年份:
    2014
  • 资助金额:
    $ 34.01万
  • 项目类别:
Bacterial cell envelope in polar positioning of autotransporter proteins
自转运蛋白极性定位中的细菌细胞包膜
  • 批准号:
    8638264
  • 财政年份:
    2014
  • 资助金额:
    $ 34.01万
  • 项目类别:
Shigella repression of innate immunity early during infection
志贺氏菌在感染早期抑制先天免疫
  • 批准号:
    8853815
  • 财政年份:
    2014
  • 资助金额:
    $ 34.01万
  • 项目类别:
Shigella repression of innate immunity early during infection
志贺氏菌在感染早期抑制先天免疫
  • 批准号:
    8772174
  • 财政年份:
    2014
  • 资助金额:
    $ 34.01万
  • 项目类别:
The cellular filopodia mechanism in Shigella membrane protrusion formation
志贺氏菌膜突起形成的细胞丝状伪足机制
  • 批准号:
    8607891
  • 财政年份:
    2013
  • 资助金额:
    $ 34.01万
  • 项目类别:
The cellular filopodia mechanism in Shigella membrane protrusion formation
志贺氏菌膜突起形成的细胞丝状伪足机制
  • 批准号:
    8430385
  • 财政年份:
    2013
  • 资助金额:
    $ 34.01万
  • 项目类别:
Career Development in Biodefense
生物防御职业发展
  • 批准号:
    8233445
  • 财政年份:
    2011
  • 资助金额:
    $ 34.01万
  • 项目类别:
Host factors in Shigella flexneri infection
福氏志贺菌感染的宿主因素
  • 批准号:
    10197816
  • 财政年份:
    2010
  • 资助金额:
    $ 34.01万
  • 项目类别:

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由两类细菌肌动蛋白 MreB 驱动的新型运动系统
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Cytoplasmic Actins in Maintenance of Muscle Mitochondria
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研究肌动蛋白和微管如何协调及其相关性。
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    19390048
  • 财政年份:
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拟南芥生殖肌动蛋白的抑制
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  • 财政年份:
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Suppression of Arabidopsis Reproductive Actins
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肌动蛋白和肌动蛋白结合蛋白的结构/相互作用
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