STRUCTURAL STUDIES OF THE BACTERIORHODOPSIN PHOTOCYCLE

细菌视紫红质光循环的结构研究

• 批准号: 6526218
• 负责人: HARTMUT LUECKE
• 金额: $ 22.16万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6526218
• 关键词: X ray crystallography; bacteriorhodopsins; chloride ion; conformation; hydrogen transport; ion transport; photochemistry; protein structure function; retinaldehyde; structural biology

DESCRIPTION (Adapted from abstract): Bacteriorhodopsin is a light-driven proton pump that has several well-characterized intermediates in its photocycle. The general aim of this proposal is to define the mechanism of proton transport in bacteriorhodopsin by mapping the conformational changes during the photocycle in atomic detail. The specific aims are 1) to determine the high resolution structure of light-adapted (ground state) bacteriorhodopsin, 2) determine the structure of the M intermediate by solving the structure of D96N bacteriorhodopsin 3) determine the crystal structure of the K intermediate, 4) solve the crystal structure of the L intermediate and 5) solve the structure of a chloride pump, either halorhodopsin, or D85S bacteriorhodopsin, which functions as a chloride pump.

细菌视紫红质是一种光驱动的质子 在其光循环中具有几个特征良好的中间体的泵。的 该建议的总体目标是定义质子传输的机制， 通过绘制光循环过程中的构象变化 在原子细节。具体目标是：1）确定高分辨率 光适应（基态）细菌视紫红质的结构，2）确定 通过解析D96N的结构来解析M中间体的结构 3）确定K中间体的晶体结构，4） 解析L中间体的晶体结构和5）解析 氯化物泵，盐视紫红质或D85S细菌视紫红质， 作为氯化物泵。

项目成果

Crystallization, X-ray diffraction analysis and SIRAS/molecular-replacenent phasing of three crystal forms of Anabaena sensory rhodopsin transducer.

鱼腥藻感觉视紫红质传感器三种晶型的结晶、X 射线衍射分析和 SIRAS/分子替代定相。

• DOI: 10.1107/s1744309106008359
• 发表时间: 2006
• 期刊: Acta crystallographica. Section F, Structural biology and crystallization communications
• 作者: Vogeley,Lutz;Luecke,Hartmut
• 通讯作者: Luecke,Hartmut

Structural clues to the mechanism of ion pumping in bacteriorhodopsin.

• DOI: 10.1016/s0065-3233(03)63005-6
• 发表时间: 2003
• 期刊: Advances in protein chemistry
• 作者: H. Luecke;J. Lanyi