GENETIC ANALYSIS OF FETAL GERM CELL DEVELOPMENT

胎儿生殖细胞发育的遗传分析

• 批准号: 6521282
• 负责人: JAMES L RESNICK
• 金额: $ 18.54万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2004-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6521282
• 关键词: biological signal transduction; cell migration; cell proliferation; chemoattractants; developmental genetics; genetically modified animals; germ cells; growth factor receptors; homozygote; inhibin; laboratory mouse; transfection /expression vector; transforming growth factors

In mammalian embryos, primordial germ cells (PGCs) are first allocated into extraembryonic tissues and subsequently proliferate while migrating to the fetal gonad. Shortly after colonizing the gonad, germ cells cease dividing and undergo sex specific differentiation. Mice harboring mutations in key growth factors or receptors exhibit PGC defects, demonstrating the necessity of somatic factors for PGC development. Using a cell culture assay which supports several aspects of PGC development, we found that germ cell proliferation can also be inhibited by two related ligands, TGFbeta and activin. The goal of this proposal is to test the hypothesis that these signaling pathways control the proliferation of PGCs in vivo, and thereby contribute to determining the final number of germ cells per embryo. Our first specific aim is to determine whether these signaling pathways individually regulate PGC proliferation. We will measure germ cell proliferation both in vivo and in culture from embryos lacking either activin or TGFbeta receptors. These two receptor systems share a common signal transduction pathway, suggesting that germ cell defects in embryos lacking one receptor system may be functionally compensated by the other system. Therefore, our second specific aim to measure PGC proliferation in embryos lacking both receptors systems in order to fully expose potential defects. Alternatively, these signaling pathways may not be essential for the normal regulation of PGC accumulation, but serve only to inhibit the proliferation of a highly multipotent and migratory cell type in ectopic locations. As a test of this hypothesis, in our third specific aim we propose to use targeted expression of TGFbeta in vivo to determine whether this ligand can prematurely inhibit PGC proliferation. Previous work has shown that PGCs migrate toward a source of TGFbeta in vitro, suggesting that these signaling pathways are part of the mysterious process that guide PGCs to the fetal gonad. Our fourth specific aim is to take advantage of a cell autonomous germ cell marker to determine whether PGCs lacking these receptor systems accumulate in ectopic locations.

在哺乳动物胚胎中，原始生殖细胞（PGCs）首先被分配到胚外组织中，随后在迁移到胎儿性腺的同时增殖。 在生殖腺定植后不久，生殖细胞停止分裂并进行性别特异性分化。 在关键生长因子或受体中携带突变的小鼠表现出PGC缺陷，证明了体细胞因子对PGC发展的必要性。 使用支持PGC发展的几个方面的细胞培养测定，我们发现生殖细胞增殖也可以被两个相关配体TGF β和激活素抑制。 该提案的目的是检验这些信号通路控制体内PGC增殖的假设，从而有助于确定每个胚胎生殖细胞的最终数量。 我们的第一个具体目标是确定这些信号通路是否单独调节PGC增殖。 我们将在体内和培养缺乏激活素或TGF β受体的胚胎中测量生殖细胞增殖。 这两个受体系统共享一个共同的信号转导途径，这表明缺乏一个受体系统的胚胎中的生殖细胞缺陷可能在功能上被另一个系统补偿。因此，我们的第二个具体目标是测量缺乏两种受体系统的胚胎中的PGC增殖，以充分暴露潜在的缺陷。 或者，这些信号传导途径可能不是PGC积累的正常调节所必需的，而是仅用于抑制异位位置中高度多能和迁移细胞类型的增殖。作为对这一假设的检验，在我们的第三个具体目标中，我们建议使用体内TGF β的靶向表达来确定这种配体是否可以过早地抑制PGC增殖。 先前的研究表明，PGCs在体外向TGF β来源迁移，这表明这些信号通路是引导PGCs进入胎儿性腺的神秘过程的一部分。 我们的第四个具体目标是利用细胞自主生殖细胞标记物来确定缺乏这些受体系统的PGCs是否在异位位置积累。