Diagnosis of Dysplasia by Fluorescence Spectroscopy

荧光光谱诊断不典型增生

• 批准号: 6524540
• 负责人: JACQUES VAN DAM
• 金额: $ 12.17万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-25 至 2005-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6524540
• 关键词: Barretts esophagus; bioimaging /biomedical imaging; clinical research; endoscopy; fluorescence spectrometry; gastrointestinal disorder diagnosis; gastrointestinal epithelium; gastrointestinal imaging /visualization; gastrointestinal neoplasms; gastroscopy; human subject; intestinal mucosa; preneoplastic state

DESCRIPTION: (Applicant's Description) The goal of this research proposal is to provide salary support to facilitate the advancement and completion of an NCl-funded clinical research proposal. The Principal Investigator, a clinically active physician scientist, is a collaborator on NCI R01 CA 53717 "Real Time In Vivo Diagnosis of Dysplasia by Fluorescence." The goal of the R01 is, in part, to develop endoscope-compatible, fluorescence spectroscopy systems for the real time detection of precancerous (dysplastic) alterations in the luminal gastrointestinal tract. Both fiber optic-based contact probe techniques for localized detection and fluorescence spectral endoscope systems for wide area imaging of disease will be developed and applied clinically. Multi-wavelength excitation fluorescence and reflectance system will be used to characterize the optical/spectroscopic properties of relevant tissue types. The results of this study will be used to select optimal excitation wavelength(s) and design fiber probes with controllable sampling depth for targeting detection of superficial lesions. By combining this information with tissue optical parameters, models of colon and esophageal fluorescence measured at colonoscopy and gastroscopy respectively, will be developed. Inverse modeling will be developed for extracting histopathological information from the clinical spectra. The existing fluorescence imaging colonoscope will be modified for additional clinical studies, including application in patients with Barrett's esophagus. The techniques developed in this program will be clinically tested for rapid detection of colorectal dysplasia/carcinoma in chronic ulcerative colitis and dysplasia in Barrett's esophagus and as such are "translational" in nature. Based on extremely successful preliminary data, light (white light) scattering spectroscopy (LSS) will be used to determine the size and degree of "crowding" of nuclei of superficial mucosal cells in the columnar-lined (Barrett's) esophagus. LSS will be used to guide the endoscopic detection (and pathological grading) of mucosal dysplasia. The Principal Investigator is devoted to training clinical researchers and will continue the formalized instruction and mentoring of young clinicians so that they may successfully engage in meaningful clinical research. In this way, the Principal Investigator will help mentor the next generation of physician scientists.

描述：（申请人描述）本研究提案的目标是 提供薪金支持，以促进晋升和完成 NCI资助的临床研究提案。首席研究员a 临床活跃的医生科学家，是NCI R 01 CA 53717的合作者 “通过荧光进行的发育不良的真实的实时体内诊断。“目标的 R 01部分是为了开发内窥镜兼容的荧光光谱学 用于癌前（发育异常）改变的真实的实时检测的系统 在腔胃肠道中。 基于光纤的接触式探头 用于局部检测和荧光光谱内窥镜系统的技术 用于疾病的广域成像将被开发并应用于临床。 将采用多波长激发荧光和反射系统 以表征相关组织类型的光学/光谱特性。 本研究的结果将用于选择最佳激励 设计具有可控采样深度的光纤探头， 靶向检测浅表病变。 通过组合该信息 利用组织光学参数、结肠和食管荧光模型 分别在结肠镜检查和胃镜检查中测量。 逆建模将被开发用于提取组织病理学 来自临床光谱的信息。 现有的荧光成像 结肠镜将进行修改，以用于其他临床研究，包括 在Barrett食管患者中的应用。 技术发展于 该程序将进行临床试验，用于快速检测结直肠癌 慢性溃疡性结肠炎中的异型增生/癌和Barrett's异型增生 食管，因此本质上是“平移的”。 采用非常 光（白色光）散射光谱（LSS）初步数据成功 将用于确定核的大小和“拥挤”程度， 柱状排列（Barrett's）食管中的浅表粘膜细胞。 LSS 将用于指导内窥镜检测（和病理分级） 粘膜发育不良 主要研究者致力于培训临床 研究人员，并将继续正规化的指导和指导， 年轻的临床医生，使他们能够成功地从事有意义的临床 research. 通过这种方式，首席研究员将帮助指导下一个 一代医学科学家。