Diagnosis of Dysplasia by Fluorescence Spectroscopy

荧光光谱诊断不典型增生

• 批准号: 6781067
• 负责人: JACQUES VAN DAM
• 金额: $ 12.17万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-25 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6781067
• 关键词: Barretts esophagus; bioimaging /biomedical imaging; clinical research; endoscopy; fluorescence spectrometry; gastrointestinal disorder diagnosis; gastrointestinal epithelium; gastrointestinal imaging /visualization; gastrointestinal neoplasms; gastroscopy; human subject; intestinal mucosa; preneoplastic state

DESCRIPTION: (Applicant's Description) The goal of this research proposal is to provide salary support to facilitate the advancement and completion of an NCl-funded clinical research proposal. The Principal Investigator, a clinically active physician scientist, is a collaborator on NCI R01 CA 53717 "Real Time In Vivo Diagnosis of Dysplasia by Fluorescence." The goal of the R01 is, in part, to develop endoscope-compatible, fluorescence spectroscopy systems for the real time detection of precancerous (dysplastic) alterations in the luminal gastrointestinal tract. Both fiber optic-based contact probe techniques for localized detection and fluorescence spectral endoscope systems for wide area imaging of disease will be developed and applied clinically. Multi-wavelength excitation fluorescence and reflectance system will be used to characterize the optical/spectroscopic properties of relevant tissue types. The results of this study will be used to select optimal excitation wavelength(s) and design fiber probes with controllable sampling depth for targeting detection of superficial lesions. By combining this information with tissue optical parameters, models of colon and esophageal fluorescence measured at colonoscopy and gastroscopy respectively, will be developed. Inverse modeling will be developed for extracting histopathological information from the clinical spectra. The existing fluorescence imaging colonoscope will be modified for additional clinical studies, including application in patients with Barrett's esophagus. The techniques developed in this program will be clinically tested for rapid detection of colorectal dysplasia/carcinoma in chronic ulcerative colitis and dysplasia in Barrett's esophagus and as such are "translational" in nature. Based on extremely successful preliminary data, light (white light) scattering spectroscopy (LSS) will be used to determine the size and degree of "crowding" of nuclei of superficial mucosal cells in the columnar-lined (Barrett's) esophagus. LSS will be used to guide the endoscopic detection (and pathological grading) of mucosal dysplasia. The Principal Investigator is devoted to training clinical researchers and will continue the formalized instruction and mentoring of young clinicians so that they may successfully engage in meaningful clinical research. In this way, the Principal Investigator will help mentor the next generation of physician scientists.

描述：（申请人的描述）本研究计划的目标是 提供薪资支持，以促进和完成 NCl 资助的临床研究提案。首席研究员 临床活跃的医师科学家，是 NCI R01 CA 53717 的合作者 “通过荧光实时体内诊断发育不良。” 的目标 R01 的部分目的是开发兼容内窥镜的荧光光谱 实时检测癌前（发育异常）改变的系统 在胃肠道腔内。 两种基于光纤的接触式探头 局部检测和荧光光谱内窥镜系统技术 用于疾病的广域成像将被开发并应用于临床。 将使用多波长激发荧光和反射系统 表征相关组织类型的光学/光谱特性。 这项研究的结果将用于选择最佳激励 波长并设计具有可控采样深度的光纤探头 针对浅表病变的检测。 通过结合这些信息 具有组织光学参数、结肠和食管荧光模型 将分别通过结肠镜检查和胃镜检查进行测量。 将开发逆向模型来提取组织病理学数据 来自临床光谱的信息。 现有的荧光成像 结肠镜将被修改以用于更多的临床研究，包括 巴雷特食管患者的应用。 开发的技术于 该程序将进行临床测试，以快速检测结直肠癌 慢性溃疡性结肠炎的不典型增生/癌和巴雷特氏不典型增生 食道等本质上是“平移的”。 基于极其 成功的初步数据，光（白光）散射光谱（LSS） 将用于确定原子核的大小和“拥挤”程度 柱状食管（巴雷特食管）中的表面粘膜细胞。 LSS 将用于指导内镜检测（和病理分级） 粘膜发育不良。 首席研究员致力于临床培训 研究人员并将继续进行正式的指导和指导 年轻的临床医生，以便他们能够成功地从事有意义的临床工作 研究。 通过这种方式，首席研究员将帮助指导下一个 一代医学科学家。