Molecular Evaluation Of Cobalt Sulfate Lung Neoplasms

硫酸钴肺肿瘤的分子评价

• 批准号: 6540981
• 负责人: Robert C Sills
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6540981
• 关键词: adenoma; animal genetic material tag; bronchogenic carcinoma; chemical carcinogen; chemical carcinogenesis; chemical related neoplasm /cancer; cobalt; environmental exposure; guanine nucleotide binding protein; laboratory mouse; lung alveolus; lung neoplasms; neoplasm /cancer genetics; nucleic acid sequence; point mutation; sulfates; tumor suppressor genes

Summary of Work: Cobalt sulfate induced a higher incidence of alveolar/bronchiolar adenomas and carcinomas in male and female mice exposed to various concentrations of cobalt sulfate compared to controls. DNA samples from lung neoplasms were sequenced for point mutations in the K-ras gene. Unique point mutations were detected in K-ras exon 2. Future work on this project includes determining the mechanism for the unique mutations and characterizing genetic alterations in potential tumor suppressor genes which may be relevant to human exposure. Comparisons between cobalt sulfate induced lung neoplasms and other lung neoplasms are currrently ongoing.

工作总结：与对照组相比，在暴露于不同浓度的硫酸钴的雄性和雌性小鼠中，硫酸钴诱导了更高的肺泡腺瘤/细支气管腺瘤和癌症的发生率。对来自肺肿瘤的DNA样本进行了K-ras基因点突变测序。在K-ras外显子2中检测到了独特的点突变。该项目的未来工作包括确定独特突变的机制，并表征可能与人类暴露相关的潜在抑癌基因的基因变化。目前，硫酸钴诱发的肺肿瘤与其他肺肿瘤的比较正在进行中。