Mechanisms of Adventurous-motility in Myxococcus xanthus

黄色粘球菌的冒险运动机制

• 批准号: 6551125
• 负责人: ROSA R YU
• 金额: $ 3.83万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-12-07 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6551125
• 关键词: Myxococcus; bacterial genetics; cell cell interaction; cell motility; developmental genetics; elasticity; electroporation; gene expression; gene mutation; genetic mapping; genetic regulation; polymerase chain reaction; postdoctoral investigator; posttranslational modifications; video microscopy

Embryonic development of vertebrates requires coordinated multicellular movements, but little is known about how such movements are regulated. Vertebrates are not easily subjected to intrusive experimental manipulations, so a simpler organism that displays similar behavior is better suited for this type of study. The bacterium Myxococcus xanthus serves as an ideal organism for studying multi- cellular movements, as M. xanthus cells move as communal swarms by gliding and assemble into complex multi-cellular fruiting bodies, followed by differentiation of myxospoes when nutrients are limited. Gliding motility in M. xanthus is controlled by two independent genetic systems: Adventurous (A) and Social (S). The S-system provides movement for cells in close proximity and depends on type IV pili. The A-system provides motility for isolated cells, but the mechanism remains unclear. The main goal of this proposal is to understand mechanism of A-motility by identifying the A-genes. The correlation between A- motility by identifying the A-genes. The correlation between A-motility and elasticotaxis, and coordination of the two independent motility systems in M. xanthus will also be investigated.

脊椎动物的胚胎发育需要协调的多细胞运动，但人们对这种运动是如何调控的知之甚少。脊椎动物不容易受到侵入性的实验操作，所以表现出相似行为的更简单的有机体更适合这种类型的研究。黄色粘球菌是研究多细胞运动的理想有机体，因为黄色粘球菌细胞通过滑动作为公共群体移动，并组装成复杂的多细胞子实体，随后在营养有限的情况下粘孢子分化。黄曲霉的滑行运动受两个独立的遗传系统控制：冒险型(A)和社会型(S)。S系统为邻近的细胞提供运动，并依赖于IV型菌毛。A系统为分离的细胞提供了运动性，但其机制尚不清楚。这一建议的主要目的是通过鉴定A-基因来了解A-运动的机制。通过鉴定A-基因来研究A-运动性之间的相关性。此外，还将研究黄曲霉A-运动与弹性趋向性之间的关系，以及这两个独立的运动系统之间的协调。