CORE--MONOCLONAL ANTIBODIES

核心--单克隆抗体

• 批准号: 6617290
• 负责人: Adrian R Krainer
• 金额: $ 7.89万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6617290
• 关键词: biomedical facility; monoclonal antibody

The Monoclonal Antibody Shared Resources provides a central site for the production of monoclonal antibodies by Cold Spring Harbor Cancer Center personnel. The purpose of the facility is to provide the resources and expertise to generate these reagents in a highly efficient and cost- effective manner. CSHL Cancer Center scientists provide specific antigens, and the Shared Resource personnel handle all stages of immunization, fusion, primary screening of hybridomas, cell culture, single-cell cloning, freezing and stage of hybridoma lines, and large-scale production of monoclonal antibodies. Immunization and test bleeds are coordinated with the personnel with the personnel of the Animal Shared Resource. Specialized antibody screens are coordinated with individual scientists. The Shared Resources promotes scientific and technical interactions among Cancer Center scientists and stimulates a great deal of cancer research that is heavily dependent on monoclonal antibody reagents.

单抗共享资源为冷泉港癌症中心工作人员生产单抗提供了一个中心站点。该设施的目的是提供资源和专门知识，以高效和具有成本效益的方式生产这些试剂。CSHL癌症中心的科学家提供特定的抗原，共享资源人员负责免疫、融合、杂交瘤的初步筛选、细胞培养、单细胞克隆、杂交瘤株的冷冻和阶段以及单抗的大规模生产的所有阶段。免疫接种和测试出血与动物共享资源的人员进行协调。专门的抗体筛查与个别科学家协调。共享资源促进了癌症中心科学家之间的科学和技术互动，并刺激了大量严重依赖单抗试剂的癌症研究。