Spectroscopic Diagnostic for Barrett's Dysplasia

巴雷特发育不良的光谱诊断

• 批准号: 6666929
• 负责人: STEPHEN FREDERICK FULGHUM
• 金额: $ 35.06万
• 依托单位: NEWTON LABORATORIES
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-06-04 至 2005-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6666929
• 关键词: Barretts esophagus; biomedical equipment development; cell nucleus; clinical research; computer program /software; computer system design /evaluation; diagnosis design /evaluation; endoscopy; fiber optics; gastrointestinal disorder diagnosis; gastrointestinal imaging /visualization; histopathology; human subject; method development; phantom model; spectrometry

DESCRIPTION (provided by applicant): Barrett's esophagus (BE) is an acquired condition of the lower esophagus in which the normal squamous tissue is replaced by a metaplastic columnar mucosa. BE itself is not malignant, but it is the primary risk factor for esophageal adenocarcinoma, and a significant number of patients with BE will have adenocarcinoma at the time of first endoscopy. Currently, examination for dysplasia requires numerous random biopsy samples. This work will complete the development and clinically test an optical instrument for detecting and grading Barrett's dysplasia. Light scattering spectroscopy (LSS) has already been shown to differentiate between normal and diseased tissue conditions in BE patients, but the analysis is complex so the data is analyzed after the procedure is over. The instrument developed by this work will be based on white light LSS, but it will use a special probe design and data reduction algorithm, developed by NLI, enabling real-time results. The probe design makes use of the properties of light scattering by cell nuclei and other tissue components to enable the separation and subtraction of interfering effects, and the characterization of the nuclei, based on using spectral, angular, and polarization characteristics of the scattering processes. The tissue characterization follows from the instrument's quantitative determination of the nuclear size and number density for the surface mucosal cells - two properties commonly used by pathologists. Specifically, NLI will (i) produce the probe, (ii) calculate the data base for the real-time algorithm, (iii) produce the instrument with light source, spectrometer, and embedded computer (suitable for interface with a standard clinical video endoscope), (iv) verify the instrument operation in the laboratory using standard scattering materials, (v) verify the background subtraction properties by using normal and diseased resected colon tissue, and (vi) conduct clinical studies on patients with Barrett's Esophagus.

描述（由申请方提供）：Barrett食管（BE）是一种获得性食管下部疾病，其中正常鳞状组织被化生柱状粘膜替代。BE本身不是恶性的，但它是食管腺癌的主要危险因素，并且相当数量的BE患者在首次内镜检查时将患有腺癌。目前，异型增生的检查需要大量的随机活检样本。这项工作将完成的发展和临床测试的光学仪器检测和分级巴雷特的发育不良。光散射光谱（LSS）已经被证明可以区分BE患者的正常和病变组织状况，但分析很复杂，因此数据在手术结束后进行分析。这项工作开发的仪器将基于白色光LSS，但它将使用由NLI开发的特殊探头设计和数据简化算法，从而实现实时结果。探头设计利用细胞核和其他组织成分的光散射特性，以基于使用散射过程的光谱、角度和偏振特性来实现干扰效应的分离和扣除以及细胞核的表征。组织表征来自于仪器对表面粘膜细胞的核大小和数量密度的定量测定-病理学家常用的两种性质。具体而言，NLI将（i）生产探头，（ii）计算实时算法的数据库，（iii）生产带有光源、光谱仪和嵌入式计算机的仪器（适于与标准临床视频内窥镜接口），（iv）使用标准散射材料验证实验室中的仪器操作，（v）通过使用正常和患病的切除结肠组织来验证背景减除特性，以及（vi）对患有巴雷特食管的患者进行临床研究。