Raman Probe for Bronchial Premalignant Lesions

描述(由申请人提供)：长期目标是一种实用的临床拉曼仪器，带有光纤探头，与支气管镜一起使用，用于根据癌前状态对支气管组织进行表征。该系统对于新一代支气管镜将特别有效，这些支气管镜的筛查效率通过使用自动荧光或其他光谱成像方法来增强。 由于肺癌是人类第二常见的癌症，是世界上最常见的癌症死亡原因，而且五年存活率非常低(14%)，因此，一种可以轻松与正常支气管镜检查程序一起使用的仪器将增加临床医疗价值，实现对可疑视野的实时检查，相对于单独使用白光支气管镜检查具有更高的灵敏度。这将使那些被选作活组织检查的部位产生更大的“阳性”。 第一阶段的具体目标是：(1)利用自由空间传播光束，从正常、发育不良和癌变的肺组织的体外样品中获取激发波长为830 nm的拉曼光谱。(2)生产和测试适合通过支气管镜活检通道插入的小直径、过滤的光纤拉曼探头。(3)实时数据分析算法的发展，它可以根据文献中描述的特征特征来指示被观察组织的状态。将获得体外支气管组织的基本拉曼数据，以帮助探针设计和算法开发。探针将被设计、制造并与拉曼原型仪器一起在体外支气管组织样本上使用，以与模拟计算进行比较，并论证实时在体仪器与支气管镜结合用于临床检查的可行性。

DESCRIPTION (provided by applicant): The long term objective is a practical clinical Raman instrument with a fiberoptic probe, for use with a bronchoscope, for characterizing bronchial tissue with respect to precancerous conditions. The system will be especially effective with a new generation of bronchoscopes that have their screening efficacy enhanced by use of auto-fluorescence or other spectroscopic imaging methods. Since lung cancer is the second most common cancer in humans, is the most common cause of cancer deaths in the world, and has a very low (14%) five year survival rate, an instrument that can be easily utilized with normal bronchoscopy procedures will add clinical medical value by enabling real time examination of suspicious sights, with enhanced sensitivity relative to white light bronchoscopy alone. This will enable greater yield of "positives" from those sites that are chosen for biopsy. The Specific Aims for Phase I are: (1) The acquisition of Raman spectra at an excitation wavelength of 830 nm from in-vitro samples of normal, dysplastic and cancerous lung tissue, using free-space propagating beams. (2) The production and testing of a small-diameter, filtered, fiberoptic Raman probe, suitable for insertion through the biopsy channel of a bronchoscope. (3) The development of real-time data analysis algorithms which can indicate the state of the observed tissue on the basis of characteristic signatures described in the literature. Basic Raman data will be obtained on in vitro bronchial tissue to assist probe design and algorithm development. Probes will be designed, fabricated and used with a Raman prototype instrument on in vitro bronchial tissue samples to compare with modeling calculations and demonstrate feasibility of a real-time in vivo instrument for use in conjunction with a bronchoscope in clinical examinations.