TIME RESOLVED SAXS STUDIES OF PROTEIN FOLDING

蛋白质折叠的时间分辨 SAXS 研究

• 批准号: 6658735
• 负责人: ANTHONY L FINK
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6658735
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Using time-resolved small-angle x-ray scattering (SAXS), we will measure the rate of compaction undergone by an unfolded protein when it is suddenly placed under native conditions. SAXS is the only time-resolved experimental probe which can give an overall size and shape indication of the conformational state of a protein. SAXS data may be used to determine the radius of gyration; to obtain shape information which may be inferred from the Kratky plot and the pair distribution function; and to determine the association state of a protein sample. Time-resolved SAXS measurements of cytochrome c refolding show the formation of an association state within the dead time of the stopped-flow mixer. We have shown that the degree of association can be reduced by using lower protein concentration and a higher final denaturant concentration.

使用时间分辨小角X射线散射（SAXS），我们将 测量未折叠蛋白质在以下情况下经历的压实速率： 它突然被置于自然条件下。 SAXS是唯一 时间分辨实验探针，可以给出整体尺寸， 蛋白质构象状态的形状指示。 SAXS数据 可用于确定回转半径;获得形状 可以从Kratky图推断出的信息， 分布函数;以及确定 蛋白质样品。 细胞色素c的时间分辨小角X射线散射测量 重新折叠显示了在死者体内形成了一种联合状态 停流混合器的时间。 我们已经证明， 可以通过使用较低的蛋白质浓度和 最终变性剂浓度较高。