CORE--FLOW CYTOMETRY

核心——流式细胞术

• 批准号: 6663395
• 负责人: Andrew W Taylor
• 金额: $ 13.47万
• 依托单位: SCHEPENS EYE RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6663395
• 关键词: biomedical facility; cell sorting; eye; flow cytometry; fluorescent dye /probe; vision

Abstract: Funds are requested by 30 vision scientists to support 4 research modules that facilitate and enhance interaction among the vision scientists in the various disciplines represented at the Schepens Eye Research Institute. The modules will extend endeavors of individual research programs by providing collaborative opportunities for projects in which investigators do not have expertise, funding, or technical capabilities. The modules: Morphology, Animal Resource, Laboratory Computer Application and Flow Cytometry. The Morphology Module will provide light and electron microscopy, confocal microscopy and image analysis capabilities and tissue preparation for histochemistry and in situ hybridization, thereby increasing the availability or morphologic techniques to biochemists, pharmacologists, cell and molecular biologists, and immunologists who need correlative morphologic data for their research. The module houses and maintains shared equipment for morphological work and is available to all members of the Core. The Animal Resource Module will provide expertise and assistance in animal surgical techniques and postoperative care, administration of anesthesia, drugs and medications. In addition, it will also provide for daily animal husbandry that is required to maintain our high standards for animal housing, sanitation, and veterinary care. The Laboratory Computer Applications module will provide support to individual and collaborative efforts by assisting in interfacing of microcomputers with lab equipment and by developing software for gathering, processing, and analyzing experimental data by microcomputer systems. The module will also provide assistance to projects involving image processing and analysis from several types of equipment sources. The module interacts actively with the Morphology and Flow Cytometry Modules supporting computer- based technology. The Flow Cytometry Module is a centralized service providing principal investigators and collaborators access to equipment and technical support for flow cytometry and cell sorting. Available to the investigators are the Coulter Epics XL flow cytometer and the Coulter ELITE EPICS ESP fluorescent cell sorter. The modules are staffed by personnel with specialized training in the respective fields and equipment. Each module is under the immediate supervision of an established, experienced investigator(s). The module heads constitute the Core Grant Committee, which is responsible to the Director of Research.

摘要：30位视觉科学家申请资助4个研究模块，以促进和加强Schepens眼科研究所各学科视觉科学家之间的互动。这些模块将通过为研究人员没有专业知识、资金或技术能力的项目提供合作机会，扩展个人研究项目的努力。课程模块：形态学、动物资源、实验室计算机应用和流式细胞术。形态学模块将提供光学和电子显微镜，共聚焦显微镜和图像分析能力以及组织化学和原位杂交的组织制备，从而增加生物化学家，药理学家，细胞和分子生物学家以及需要相关形态学数据进行研究的免疫学家的形态学技术的可用性。模块容纳和维护形态学工作的共享设备，并可供所有核心成员使用。动物资源模块将在动物手术技术和术后护理、麻醉管理、药物和药物方面提供专业知识和协助。此外，它还将提供日常畜牧业，以保持我们对动物住房，卫生和兽医护理的高标准。实验室计算机应用模块将通过协助微型计算机与实验室设备的接口，并通过开发用于收集、处理和分析微型计算机系统实验数据的软件，为个人和协作的努力提供支持。该模块还将为涉及图像处理和分析的项目提供协助，这些项目来自几种类型的设备来源。该模块与支持计算机技术的形态学和流式细胞术模块积极交互。流式细胞术模块是一项集中服务，为主要研究人员和合作者提供流式细胞术和细胞分选的设备和技术支持。可供研究人员使用的是Coulter Epics XL流式细胞仪和Coulter ELITE Epics ESP荧光细胞分选仪。这些模块由在各自领域和设备方面受过专门培训的人员组成。每个模块都是在一个成熟的，经验丰富的调查员的直接监督下。各模块主管组成核心拨款委员会，向研究部主任负责。