EMBRYONIC DEVELOPMENT OF THE EFFERENT SYSTEM

传出系统的胚胎发育

• 批准号: 6564033
• 负责人: BERND FRITZSCH
• 金额: $ 26.25万
• 依托单位: FATHER FLANAGAN'S BOYS' HOME
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-02-01 至 2003-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564033
• 关键词: axon; cell migration; chick embryo; developmental neurobiology; efferent nerve; fluorescent dye /probe; gene expression; innervation; laboratory mouse; motor neurons; neuroanatomy; neurogenesis; neuronal guidance; neurotrophic factors; vertebrate embryology

The efferent motoneuron system that projects to the ear develops in mice and chicken out of the facial branchial motor primordium. Like other motor systems, the efferent cells will likely depend for their survival on unknown, neurotrophic factor(s) available at their target. In addition, various conditions such as the time of arrival and the size of the target area innervated have been suggested to play a role in the survival of motoneurons. This project seeks to test this notion by quantifying the numbers of efferents projecting to the ear over time. We will use certain features unique to the efferent system to explore issues of timing of arrival and target size which is different in the various subpopulations of efferents, and which cannot be investigated in any other motor system. We will also explore mutants with variable reductions of both the afferent and efferent innervation to certain sensory epithelia or reduction of the ear, including surgical removal, to analyze further the quantitative relationship between the developing ear and the developing efferents. Our experiments will also provide insights into the possibly limited availability of trophic support only at the otocyst by testing the survival of efferent cells rerouted to a non-otic target in chickens. Together this information will provide baseline data that can help to find clues for interventive measures in patients with loss of hair cells to prevent loss of efferents.

投射到耳朵的传出运动神经元系统在小鼠中发育 和鸡的面部鳃运动原基。 像其他 运动系统，传出细胞可能会依赖于它们的生存 在未知的，神经营养因子（S）可在其目标。 在 此外，各种条件，如到达时间和大小 的靶区神经支配已被建议发挥作用，在 运动神经元的存活。 本项目旨在通过以下方式测试这一概念： 随着时间的推移量化投射到耳朵的传出神经的数量。 我们将使用传出系统特有的某些特征来探索 到达时间和目标大小的问题，这是不同的， 传出神经的各种亚群，并且无法进行研究 在任何其他电机系统中。 我们还将探索具有可变 减少传入和传出神经支配的某些 感觉上皮或耳的缩小，包括手术切除， 进一步分析了发展中国家与发展中国家之间的数量关系， 耳和发育中的传出神经。 我们的实验还将提供 对营养支持可能有限的见解 通过测试传出细胞的存活率， 鸡的非耳靶点。 这些信息将提供 基线数据可以帮助找到干预措施的线索， 毛细胞丢失的患者，以防止传出神经丢失。