Chlamydia pneumoniae vaccine candidates

描述（由申请人提供）：衣原体（C.）肺炎是一种主要的病原体 社区获得性上呼吸道和下呼吸道感染和肺炎。 越来越多的证据表明C.肺炎感染在 在发达国家动脉粥样硬化性冠心病中的作用， C.肺炎是一个主要的公共卫生问题。这显然值得努力， 研制出抗C.肺炎的预防或治疗 呼吸系统疾病，可能还有冠心病和动脉粥样硬化。 使用我们新的基因免疫技术，我们能够将 将病原体的基因组转化为最佳候选疫苗，最近， 在C.鹦鹉热感染，我们发现10 保护性基因也保护了原始宿主动物。仔细阅读 C.全基因组序列肺炎，我们建议作为第一步， C. pneumoniae疫苗i）检测C.肺炎同源物 保护C psittaci基因在C. 肺炎呼吸道感染; ii）进行C.肺炎全基因组 寻找介导预防性免疫的最佳抗原， 呼吸道感染; iii）进行实验以了解 这种免疫干预的成功机制。我们建议 具体目标如下：1）检测C. 10种肺炎链球菌同源物 保护C psittaci基因在小鼠预防性呼吸道感染C. 肺炎感染。2)筛选所有约1，000 C。pneumoniae基因 它们在小鼠预防模型中的保护功效。3)了解 在远交人群中可能的反应谱，剖析 免疫机制的疾病保护介导的C。肺炎 使用几种近交系的呼吸道疾病模型中的疫苗候选蛋白 小鼠品系。

DESCRIPTION (Provided by Applicant): Chlamydia (C.) pneumoniae is a major agent of community-acquired upper and lower respiratory infection and pneumonia. Increasing evidence suggests that C. pneumoniae infection plays an integral role in atherosclerotic coronary heart disease in developed countries, making C. pneumoniae a major public health concern. This clearly merits an effort to develop a vaccine against C. pneumoniae for prevention or treatment of respiratory disease, and possibly coronary heart disease and atherosclerosis. Using our new genetic immunization technologies, we are able to deconvolute the genomes of pathogens into the best vaccine candidates, and have recently validated this in a mouse model of C. psittaci infection in which we found 10 protective genes that also protect the original host animal. Perusing the complete genome sequence of C. pneumoniae, we propose as first step towards a C. pneumoniae vaccine to i) examine the C. pneumoniae homologs of the protective C. psittaci genes for protective efficacy in a mouse model of C. pneumoniae respiratory infection; ii) conduct a C. pneumoniae genome-wide search for the best antigens mediating prophylactic immunity against respiratory infection; and iii) perform experiments to understand the mechanisms for the success of such immunological intervention. We propose the following specific aims: 1) Test the C. pneumoniae homologs of the 10 protective C. psittaci genes in a mouse prophylactic respiratory model of C. pneumoniae infection. 2) Screen all approximately 1,000 C. pneumoniae genes for their protective efficacy in the mouse prophylactic model. 3) To understand the spectrum of possible responses in an outbred human population, dissect the immunological mechanisms of disease protection mediated by the C. pneumoniae vaccine candidate proteins in respiratory disease models using several inbred mouse strains.