Chlamydia pneumoniae vaccine candidates

肺炎衣原体候选疫苗

• 批准号: 6619455
• 负责人: BERNHARD KALTENBOECK
• 金额: $ 56.74万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6619455
• 关键词: Chlamydiaceae; Chlamydophila psittaci; bacterial DNA; bacterial vaccines; chlamydial disease; disease /disorder model; laboratory mouse; molecular cloning; respiratory infections; vaccine development; vector vaccine

DESCRIPTION (Provided by Applicant): Chlamydia (C.) pneumoniae is a major agent of community-acquired upper and lower respiratory infection and pneumonia. Increasing evidence suggests that C. pneumoniae infection plays an integral role in atherosclerotic coronary heart disease in developed countries, making C. pneumoniae a major public health concern. This clearly merits an effort to develop a vaccine against C. pneumoniae for prevention or treatment of respiratory disease, and possibly coronary heart disease and atherosclerosis. Using our new genetic immunization technologies, we are able to deconvolute the genomes of pathogens into the best vaccine candidates, and have recently validated this in a mouse model of C. psittaci infection in which we found 10 protective genes that also protect the original host animal. Perusing the complete genome sequence of C. pneumoniae, we propose as first step towards a C. pneumoniae vaccine to i) examine the C. pneumoniae homologs of the protective C. psittaci genes for protective efficacy in a mouse model of C. pneumoniae respiratory infection; ii) conduct a C. pneumoniae genome-wide search for the best antigens mediating prophylactic immunity against respiratory infection; and iii) perform experiments to understand the mechanisms for the success of such immunological intervention. We propose the following specific aims: 1) Test the C. pneumoniae homologs of the 10 protective C. psittaci genes in a mouse prophylactic respiratory model of C. pneumoniae infection. 2) Screen all approximately 1,000 C. pneumoniae genes for their protective efficacy in the mouse prophylactic model. 3) To understand the spectrum of possible responses in an outbred human population, dissect the immunological mechanisms of disease protection mediated by the C. pneumoniae vaccine candidate proteins in respiratory disease models using several inbred mouse strains.

描述（由申请人提供）：肺炎衣原体 (C.) 是主要病原体 社区获得性上、下呼吸道感染和肺炎。 越来越多的证据表明，肺炎衣原体感染起着不可或缺的作用。 在发达国家动脉粥样硬化性冠心病中的作用 肺炎衣原体是一个主要的公共卫生问题。这显然值得我们努力 开发针对肺炎衣原体的疫苗来预防或治疗 呼吸系统疾病，可能还有冠心病和动脉粥样硬化。 利用我们新的基因免疫技术，我们能够解开 将病原体基因组转化为最佳候选疫苗，最近 在鹦鹉热衣原体感染的小鼠模型中验证了这一点，我们在其中发现了 10 保护基因也保护原始宿主动物。细读 肺炎衣原体的完整基因组序列，我们建议作为迈向 肺炎衣原体疫苗 i) 检查肺炎衣原体同源物 保护性鹦鹉热鹦鹉热基因在鹦鹉热鹦鹉热鹦鹉热小鼠模​​型中具有保护功效。 肺炎呼吸道感染; ii) 进行肺炎衣原体全基因组研究 寻找介导预防性免疫的最佳抗原 呼吸道感染； iii) 进行实验以了解 这种免疫干预的成功机制。我们建议 具体目标如下： 1) 检测 10 种肺炎衣原体的同源物 鹦鹉热念珠菌小鼠预防性呼吸道模型中的保护性鹦鹉热念珠菌基因。 肺炎感染。 2) 筛选所有大约 1,000 个肺炎衣原体基因 它们在小鼠预防模型中的保护功效。 3）了解 远交人群中可能的反应范围，剖析 肺炎衣原体介导的疾病保护的免疫学机制 使用几种近交系在呼吸道疾病模型中开发疫苗候选蛋白 小鼠品系。