Chlamydia pneumoniae vaccine candidates

肺炎衣原体候选疫苗

• 批准号: 6750098
• 负责人: BERNHARD KALTENBOECK
• 金额: $ 21.64万
• 依托单位: AUBURN UNIVERSITY AT AUBURN
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6750098
• 关键词: Chlamydiaceae; Chlamydophila psittaci; bacterial DNA; bacterial vaccines; chlamydial disease; disease /disorder model; laboratory mouse; molecular cloning; respiratory infections; vaccine development; vector vaccine

DESCRIPTION (Provided by Applicant): Chlamydia (C.) pneumoniae is a major agent of community-acquired upper and lower respiratory infection and pneumonia. Increasing evidence suggests that C. pneumoniae infection plays an integral role in atherosclerotic coronary heart disease in developed countries, making C. pneumoniae a major public health concern. This clearly merits an effort to develop a vaccine against C. pneumoniae for prevention or treatment of respiratory disease, and possibly coronary heart disease and atherosclerosis. Using our new genetic immunization technologies, we are able to deconvolute the genomes of pathogens into the best vaccine candidates, and have recently validated this in a mouse model of C. psittaci infection in which we found 10 protective genes that also protect the original host animal. Perusing the complete genome sequence of C. pneumoniae, we propose as first step towards a C. pneumoniae vaccine to i) examine the C. pneumoniae homologs of the protective C. psittaci genes for protective efficacy in a mouse model of C. pneumoniae respiratory infection; ii) conduct a C. pneumoniae genome-wide search for the best antigens mediating prophylactic immunity against respiratory infection; and iii) perform experiments to understand the mechanisms for the success of such immunological intervention. We propose the following specific aims: 1) Test the C. pneumoniae homologs of the 10 protective C. psittaci genes in a mouse prophylactic respiratory model of C. pneumoniae infection. 2) Screen all approximately 1,000 C. pneumoniae genes for their protective efficacy in the mouse prophylactic model. 3) To understand the spectrum of possible responses in an outbred human population, dissect the immunological mechanisms of disease protection mediated by the C. pneumoniae vaccine candidate proteins in respiratory disease models using several inbred mouse strains.

描述（由申请人提供）：衣原体（C.）肺炎是一种主要的病原体 社区获得性上呼吸道和下呼吸道感染和肺炎。 越来越多的证据表明C.肺炎感染在 在发达国家动脉粥样硬化性冠心病中的作用， C.肺炎是一个主要的公共卫生问题。这显然值得努力， 研制出抗C.肺炎的预防或治疗 呼吸系统疾病，可能还有冠心病和动脉粥样硬化。 使用我们新的基因免疫技术，我们能够将 将病原体的基因组转化为最佳候选疫苗，最近， 在C.鹦鹉热感染，我们发现10 保护性基因也保护了原始宿主动物。仔细阅读 C.全基因组序列肺炎，我们建议作为第一步， C. pneumoniae疫苗i）检测C.肺炎同源物 保护C psittaci基因在C. 肺炎呼吸道感染; ii）进行C.肺炎全基因组 寻找介导预防性免疫的最佳抗原， 呼吸道感染; iii）进行实验以了解 这种免疫干预的成功机制。我们建议 具体目标如下：1）检测C. 10种肺炎链球菌同源物 保护C psittaci基因在小鼠预防性呼吸道感染C. 肺炎感染。2)筛选所有约1，000 C。pneumoniae基因 它们在小鼠预防模型中的保护功效。3)了解 在远交人群中可能的反应谱，剖析 C.介导的疾病保护的免疫机制。肺炎 使用几种近交系的呼吸道疾病模型中的疫苗候选蛋白 小鼠品系。

项目成果

Acute Chlamydia pneumoniae reinfection accelerates the development of insulin resistance and diabetes in obese C57BL/6 mice.

急性肺炎衣原体再感染会加速肥胖 C57BL/6 小鼠胰岛素抵抗和糖尿病的发展。

• DOI: 10.1086/599796
• 发表时间: 2009
• 期刊: The Journal of infectious diseases
• 作者: Wang,Chengming;Gao,Dongya;Kaltenboeck,Bernhard
• 通讯作者: Kaltenboeck,Bernhard

One-step real-time duplex reverse transcription PCRs simultaneously quantify analyte and housekeeping gene mRNAs.

一步式实时双链逆转录 PCR 可同时定量分析物和管家基因 mRNA。

• DOI: 10.2144/04363rn06
• 发表时间: 2004
• 期刊: BioTechniques
• 影响因子: 2.7
• 作者: Wang,Chengming;Gao,Dongya;Vaglenov,Alexander;Kaltenboeck,Bernhard
• 通讯作者: Kaltenboeck,Bernhard

Rapid high-yield mRNA extraction for reverse-transcription PCR.

快速高产量 mRNA 提取用于反转录 PCR。

• DOI: 10.1016/j.jbbm.2006.10.003
• 发表时间: 2007
• 期刊: Journal of biochemical and biophysical methods
• 作者: Wang,Chengming;Kim,Teayoun;Gao,Dongya;Vaglenov,Alexander;Kaltenboeck,Bernhard
• 通讯作者: Kaltenboeck,Bernhard

Temporal delay of peak T-cell immunity determines Chlamydia pneumoniae pulmonary disease in mice.

T 细胞免疫峰值的时间延迟决定了小鼠肺炎衣原体肺部疾病。

• DOI: 10.1128/iai.00569-08
• 发表时间: 2008
• 期刊: Infection and immunity
• 影响因子: 3.1
• 作者: Wang,Chengming;vanGinkel,FrederikW;Kim,Teayoun;Li,Dan;Li,Yihang;Dennis,JohnC;Kaltenboeck,Bernhard
• 通讯作者: Kaltenboeck,Bernhard

Novel Chlamydia pneumoniae vaccine candidates confirmed by Th1-enhanced genetic immunization.

Th1 增强基因免疫证实了新型肺炎衣原体候选疫苗。

• DOI: 10.1016/j.vaccine.2009.11.046
• 发表时间: 2010
• 期刊: Vaccine
• 影响因子: 5.5
• 作者: Li,Yihang;Ahluwalia,SudhirK;Borovkov,Alexandre;Loskutov,Andrey;Wang,Chengming;Gao,Dongya;Poudel,Anil;Sykes,KathrynF;Kaltenboeck,Bernhard
• 通讯作者: Kaltenboeck,Bernhard