Mussel-Inspired Protective Coatings

受贻贝启发的防护涂层

• 批准号: 6687100
• 负责人: JOHN HERBERT WAITE
• 金额: $ 31.98万
• 依托单位: UNIVERSITY OF CALIFORNIA SANTA BARBARA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-07-02 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6687100
• 关键词: Bivalvia; biomechanics; biomimetics; granule; iron; polymerase chain reaction; protein localization; protein structure function; scanning electron microscopy; surface coating; transmission electron microscopy

DESCRIPTION (provided by applicant): Mussels produce externalized tendons called byssal threads, which collectively serve as holdfasts. Byssal threads are coated with a thin protective cuticle (5-20 qm) that shields against abrasion by sand, chemical erosion, and microbial degradation, yet is flexible enough to accommodate strains of 0.7 by the fibrous byssal core. Mfp-1 is the primary structural protein of the byssal coating. It is also used commercially as a universal cell and tissue attachment factor and is being developed as a biomimetic tether for polymer attachment to surfaces. The present proposal aims to better understand the disposition, properties, and processing of mfp-1 in the byssal coating. This is considered essential for the intelligent synthesis and use of analogues for biomedical materials applications. Research aims include 1) the immunolocalization of mfp-1 in the mottled granules of the coating and foot; 2) mapping the elemental composition with the nanomechanical properties of the coating; measurement of metal binding affinities of fp-ls from four different mussel species (Mytilus galloprovincialis, M. californianus, Modiolus modiolus and Septifer bifurcatus) using an electrochemical chelate scale, and 4) investigating metal-induced collapse kinetics in adsorbed fp-ls by quartz crystal microbalance analysis in dissipation mode. In years 3 and 4, biomimetic coatings comprised of mfp-1 with and without added metal will be fabricated in vitro by solvent evaporation, coacervation, and controlled self-assembly processes. All films will be evaluated by macro- and nanomechanical tests, and their resistance to a diverse range of biochemical, thermal, and mechanical insults will be examined.

描述（由申请人提供）：贻贝产生外部化的肌腱，称为byssys线程，共同作为holdfasts。 深海丝被一层薄薄的保护层（5-20 qm）覆盖，可以防止沙子的磨损、化学侵蚀和微生物降解，但它的柔韧性足以适应纤维状深海芯的0.7应变。Mfp-1是深海鱼的主要结构蛋白。 它也在商业上用作通用的细胞和组织附着因子，并正在开发作为聚合物附着到表面的仿生系链。 本研究的目的是更好地了解mfp-1在深海涂层中的分布、性质和加工过程。 这被认为是必不可少的智能合成和使用类似物的生物医学材料的应用。 研究目的包括：1）在膜和足的斑驳颗粒中mfp-1的免疫定位; 2）绘制膜的元素组成与纳米机械性质;测量来自四种不同贻贝物种（贻贝（Mytilus galloprovincialis，M. californianus，Modiolus modiolus和Septifer bifurcatus），以及4）通过石英晶体微天平分析在耗散模式下研究吸附的fp-ls中金属诱导的塌陷动力学。 在第3年和第4年，将通过溶剂蒸发、凝聚和可控自组装过程在体外制备由mfp-1和添加或不添加金属组成的仿生涂层。 所有的电影将通过宏观和纳米力学测试进行评估，并将检查其对各种生物化学，热和机械损伤的抵抗力。