MACCHESS CONSORTIUM FOR PHASING METHODS IN MACROMOLECULAR CRYSTALLOGRAPHY

MACCHESS 高分子晶体学定相方法联盟

• 批准号: 6667787
• 负责人: STEVEN E EALICK
• 金额: $ 14.27万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2003-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6667787
• 关键词: bioimaging /biomedical imaging; biological products; biomedical equipment development; biomedical resource; proteins; radiography; structural biology

MacCHESS has implemented five CCD-based x-ray detectors; a Princeton 1k detector, the Princeton Scientific Instruments 2k detector, an Area Detector Systems Corporation (ADSC) 1k detector and two ADSC Quantum 4 mosaic detectors. As part of the process of testing and calibrating these detectors we routinely measure data using the various CCD detector systems. During the past year the two Quantum 4 detectors were installed and debugged and are now routinely available for routine data collection. As part of the installation, a detector mount was developed that provide for a two-theta offset. During the past year we have developed a local calibration system based on a portable x-ray generator. In addition, we found that the DENZO data processing package did not support the Quantum 4 detector and established a collaboration to develop a separate software package using MOSFLM as the integration program. All of these developments have reached a stage where users can routinely collect and process data. All of these developments were first tested using crystals for which we have a great deal of experience. These include bovine purine nucleoside phosphorylase (cubic) and E.coli purine nucleoside phosphorylase (hexagonal) which we routinely use for testing detector performance. Both crystals are reproducible, diffract to high resolution, freeze well and have high symmetry. Therefore, they provide excellent test samples for detector testing. In the future we will test a 2k CCD module from ADSC that will serve as the basis of a 2k version of the Quantum 4. This detector will have an active area of 20 cm x 20 cm and 16 million pixels.

MacChess已实施了五个基于CCD的X射线探测器；一个 普林斯顿1K探测器，普林斯顿科学仪器2K 检测器，区域探测器系统公司（ADSC）1K检测器和 两个ADSC量子4马赛克探测器。 作为过程的一部分 测试和校准这些检测器，我们通常测量数据 使用各种CCD检测器系统。 在过去的一年中 量子4检测器已安装和调试，现在定期进行 可用于常规数据收集。 作为安装的一部分， 开发了检测器支架，可提供两秒钟的偏移。 在过去的一年中，我们开发了当地的校准系统 基于便携式X射线发电机。 此外，我们发现 Denzo数据处理软件包不支持量子4检测器 并建立了一个合作来开发单独的软件包 使用MOSFLM作为集成计划。 所有这些发展 已经到达了一个阶段，用户可以定期收集和处理 数据。 所有这些发展首先使用晶体进行测试 我们有很多经验。 这些包括牛嘌呤 核苷磷酸化酶（立方）和大肠杆菌嘌呤核苷 我们通常用于测试检测器的磷酸化酶（六边形） 表现。 两种晶体都是可重复的，衍射到高 分辨率，冷冻良好并具有高对称性。 因此，他们 为检测器测试提供出色的测试样品。 将来我们 将从ADSC测试2K CCD模块，该模块将作为 量子4的2K版本。该检测器将具有一个活性区域 20厘米x 20厘米和1600万像素。