NE-CAT: A Resource for Advanced Macromolecular Crystallography

NE-CAT：高级高分子晶体学资源

• 批准号: 10379339
• 负责人: STEVEN E EALICK
• 金额: $ 277.31万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10379339
• 关键词: Advisory Committees; Aging; Area; Automatic Data Processing; Biological; Biological Process; Biomedical Research; Communities; Computer software; Crystallography; Data Analyses; Data Collection; Development; Drug Design; Drug Targeting; Education and Outreach; Educational workshop; Ensure; Funding; Goals; Grouping; Hand; Healthcare; Individual; Infrastructure; Institution; Laboratories; Logistics; Mainstreaming; Maintenance; Manuscripts; Modification; Optics; Paper; Persons; Pharmacologic Substance; Photons; Productivity; Professional Organizations; Publications; Publishing; Recruitment Activity; Research; Research Personnel; Research Project Grants; Resources; Robotics; Roentgen Rays; Running; Sampling; Schedule; Site; Source; Structure; Synchrotrons; Techniques; Technology; Time; Training; Training Activity; Training Programs; United States National Institutes of Health; Universities; Work; beamline; biological systems; cohesion; computerized data processing; data structure; design; detector; experience; improved; macromolecule; meetings; member; operation; outreach; outreach program; programs; repaired; three dimensional structure; web based interface; web site

The main goal of the NE-CAT Resource is to provide user access to state-of-the-art synchrotron beamlines for macromolecular crystallography. NE-CAT began planning and design for two beamlines in 2001. Beamline 24- ID-C became available to the Advanced Photon Source users in 2006 and beamline 24-ID-E became available in 2007. During the ensuing years, beamline improvements have resulted in ultrastable, small X-ray beams ideally suited for challenging problems in macromolecular crystallography. Each beamline is equipped with a high capacity sample automounter and a microdiffractometer. Beamline 24-ID-C features a PILATUS 6MF pixel array detector, and by early 2017 an EIGER 16M pixel array detector will be available on beamline ID-24- E. NE-CAT provides automated software for data collection and analysis (RAPD) and a Web-based interface for remote data collection. We will continue to provide routine maintenance to ensure efficient and reliable data collection, and we propose an upgrade program to maintain state-of-the-art capabilities over the five years covered by this application. NE-CAT has an experienced and dedicated scientific staff who enable access to the beamlines. The staff is available or on call 24/7 to assist users with data collection and processing needs. This highly regarded staff is a major strength of NE-CAT. NE-CAT has an outstanding record of reliability with technical problems rarely interfering with user access. NE-CAT is directed by Dr. Steven Ealick, who has more that 25 years of experience overseeing the operation of synchrotron facilities for macromolecular crystallography. The technical group, led by Deputy Director Dr. Malcolm Capel, and the operations group, led by Associate Director Dr. Kanagalaghatta Rajashankar work closely together to ensure that the needs of the user community are met. Assistant Director Dr. Frank Murphy is responsible for development and maintenance of RAPD. Together, these four individuals make up the NE-CAT management team. NE-CAT beam time is in high demand, and each run all available beamtime is allocated to users. NE-CAT typically hosts more than 1500 users per year from about 160 unique user groups, either on site or remotely. NE-CAT users published about 150 papers during the last year, and they are projected to publish around 170 in 2016. NE-CAT's record of productivity places us among the top of worldwide facilities for macromolecular crystallography. NE-CAT operates an extensive user training program adaptable to first time users, advanced users with technically challenging problems, and everyone in between. A Web site is available to provide all information needed by new or current users. NE-CAT participates in many training and outreach activities including organization of an annual workshop and participation in professional society meetings. The staff works closely with user groups and are often invited to coauthor manuscripts resulting from user assistance.

NE-CAT资源的主要目标是为用户提供对最先进的同步加速器光束线的访问 大分子结晶学。NE-CAT于2001年开始规划和设计两条光束线。光束线24- ID-C在2006年向高级光子源用户开放，BEAMLINE 24-ID-E也可用 2007年。在接下来的几年里，光束线的改进导致了超稳定的小X射线光束 非常适合于大分子结晶学中具有挑战性的问题。每条光束线都配备了一个 大容量样品自动安装机和显微衍射仪。Beamline 24-ID-C配备Pilatus 6MF 像素阵列探测器，到2017年初，Eiger 16M像素阵列探测器将在光束线ID-24上提供- E.NE-CAT提供自动数据收集和分析软件(RAPD)和基于Web的界面 用于远程数据收集。我们将继续提供例行维护，以确保数据高效可靠 我们提出了一项升级计划，以在五年内保持最先进的能力 此应用程序涵盖的。NE-CAT拥有经验丰富且敬业的科学工作人员，他们能够访问 光束线。工作人员全天候或随叫随到，以帮助用户满足数据收集和处理需求。 这些备受推崇的员工是NE-CAT的一大优势。NE-CAT拥有出色的可靠性记录， 技术问题很少会干扰用户访问。NE-CAT由史蒂文·伊利克博士执导，他有更多 25年监督大分子同步加速器设备运行的经验 结晶学。由副主任马尔科姆·卡佩尔博士领导的技术小组和运营小组领导 由副主任Kanagalaghatta Rajashankar博士密切合作，确保 满足了用户群体的需求。助理主任Frank Murphy博士负责开发和维护 对随机扩增多态性DNA进行了分析。这四个人一起组成了NE-CAT管理团队。Ne-CAT波束时间到了 要求很高，并且每次运行所有可用的波束时间都分配给用户。Ne-cat通常拥有超过 每年有1500名用户，来自现场或远程的约160个独立用户组。电子猫用户名单公布 去年发表了大约150篇论文，预计2016年将发表约170篇。Ne-CAT的记录 生产效率的提高使我们跻身于全球大分子结晶学设备的顶尖行列。NE-CAT 运营广泛的用户培训计划，适用于新用户、高级用户和技术人员 挑战问题，以及介于两者之间的每个人。网站可用于提供所需的所有信息 新用户或当前用户。Ne-CAT参加了许多培训和外展活动，包括组织 年度研讨会和参加专业协会会议。工作人员与用户组密切合作 并经常被邀请共同撰写由用户帮助产生的手稿。