Pixel Array Detector for Macromolecular Crystallography

用于高分子晶体学的像素阵列检测器

• 批准号: 9074913
• 负责人: STEVEN E EALICK
• 金额: $ 200万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9074913
• 关键词: Aging; Area; Caliber; Characteristics; Crystallography; Data; Data Collection; Data Quality; Data Set; Funding; Future; Generations; Noise; Optics; Photons; Process; Productivity; Publications; Research Infrastructure; Research Project Grants; Resolution; Roentgen Rays; Sampling; Source; Speed; Synchrotrons; System; Time; United States National Institutes of Health; base; beamline; cluster computing; detector; experience; improved; programs

 DESCRIPTION (provided by applicant): The Northeastern Collaborative Access Team (NE-CAT) operates two undulator beamlines for macromolecular crystallography at Sector 24 of the Advanced Photon Source (APS). Beamline 24-ID-C is equipped with a Dectris PILATUS 6MF pixel array detector (PAD) and beamline 24-ID-E is equipped with an ADSC Q315 CCD-based detector. NE-CAT has operated for 15 years and is currently oversubscribed. More than 85% of the beam time is allocated to NIH funded research projects. During the past 12 months, over 160 publications resulted from data collected at NE-CAT. PADs became commercially available less than five years ago and are now established as the best available solution for collecting macromolecular crystallography data at synchrotron beamlines. Our PILATUS 6MF was commissioned about three years ago and its availability has revolutionized data collection at NE-CAT. Compared to CCD-based detectors, PADs have no readout noise, higher dynamic range, and much higher readout speed. These features allow shutterless data collection and diffractive alignment of small crystals embedded in opaque cryomedia, and result in improved data quality and resolution. Consequently, we are able to accommodate more users with a commensurate increase in productivity. The number of publications from beamline 24-ID-C in 2014 increased by more than 20% compared to the number of publications in 2013. Our experience with the PILATUS 6MF, the aging of our ADSC Q315 CCD detector, which is no longer supported, an upgrade of our 24-ID-E X-ray optics to produce intense 5 µm diameter X-ray beams at the sample, and the planned APS lattice upgrade, has led us to request funding for the generation PAD, the Dectris EIGER 16M. The EIGER 16M will be installed at the NE-CAT beamline 24-ID-E as a replacement for the Q315. All of the necessary infrastructure is in place including a 384 Xeon core compute cluster and 432 TB of disk storage. The EIGER 16M has smaller pixels (75 µm compared to 150 µm for the PILATUS 6MF) and a faster framing rate. The EIGER 16M provides all of the advantages of the PILATUS 6MF and more. In particular, the EIGER 16M is better suited for microbeam crystallography, which is one of our major areas of emphasis. These advantages will allow us to develop serial microcystallography in which very intense beams are used to collect one or a few frames from microcrystals. The frames are then processed and merged to create a complete dataset. In the long term, the secondary KB focusing system currently being installed at 24-ID-E in combination with the APS multi-bend achromat lattice upgrade will provide about two orders increase in beam brilliance. This is ideally matched to our future plans for our microbeam crystallography program. The EIGER 16M will further enhance our technological capabilities for a broad range of projects, many of which require the unprecedented characteristics of the EIGER 16M.

 描述（由申请人提供）：东北协作访问团队（NE-CAT）在先进光子源（APS）的第24区操作两条用于大分子晶体学的波荡器光束线。光束线24-ID-C配备有Dectris PILLENT 6 MF像素阵列检测器（PAD），光束线24-ID-E配备有ADSC Q315 CCD检测器。NE-CAT已经运营了15年，目前已经超额认购。超过85%的光束时间分配给NIH资助的研究项目。在过去的12个月里，NE-CAT收集的数据产生了160多篇出版物。PAD在不到五年前开始商业化，现在已成为在同步加速器光束线上收集大分子晶体学数据的最佳解决方案。我们的PILLENT 6 MF大约在三年前投入使用，它的可用性彻底改变了NE-CAT的数据收集。与基于CCD的探测器相比，PAD没有读出噪声，动态范围更高，读出速度更快。这些功能允许无快门的数据收集和嵌入不透明低温介质中的小晶体的衍射对准，并导致改进的数据质量和分辨率。因此，我们能够容纳更多的用户，并相应提高生产力。与2013年的出版物数量相比，2014年来自beamline 24-ID-C的出版物数量增加了20%以上。我们在PILLENT 6 MF方面的经验，ADSC Q315 CCD探测器的老化（不再支持），我们的24-ID-E X射线光学系统的升级（在样品上产生5 µm直径的强烈X射线束），以及计划中的APS晶格升级，使我们申请了PAD一代Dectris EIGER 16 M的资金。EIGER 16 M将安装在NE-CAT光束线24-ID-E上，作为Q315的替代品。所有必要的基础设施都已到位，包括384 Xeon核心计算集群和432 TB磁盘存储。EIGER 16 M的像素更小（75 µm，而PILLENT 6 MF的像素为150 µm），帧速率更快。EIGER 16 M具有PILLENT 6 MF的所有优点，甚至更多。特别是EIGER 16 M更适合微束晶体学，这是我们的主要重点领域之一。这些优点将使我们能够发展一系列的微囊造影术，其中非常强烈的光束被用来收集一个或几个帧从微晶。然后处理和合并帧以创建完整的数据集。从长远来看，目前安装在24-ID-E的次级KB聚焦系统与APS多弯曲消色差光栅升级相结合，将提供大约两个数量级的光束亮度增加。这与我们未来的微束晶体学计划非常匹配。EIGER 16 M将进一步增强我们在广泛项目中的技术能力，其中许多项目需要EIGER 16 M前所未有的特性。

项目成果

Structure and function of the two-component flavin-dependent methanesulfinate monooxygenase within bacterial sulfur assimilation

细菌硫同化中双组分黄素依赖性甲亚磺酸单加氧酶的结构和功能

• DOI: 10.1016/j.bbrc.2019.11.008
• 发表时间: 2020
• 期刊: Biochemical and Biophysical Research Communications
• 影响因子: 3.1
• 作者: Soule, Jess;Gnann, Andrew D.;Gonzalez, Reyaz;Parker, Mackenzie J.;McKenna, Kylie C.;Nguyen, Son V.;Phan, Ngan T.;Wicht, Denyce K.;Dowling, Daniel P.
• 通讯作者: Dowling, Daniel P.