Optical Intrinsic Signal Imaging of Seizure

描述（由申请人提供）：结构和 功能性神经成像在过去十年中取得了显著进展。 然而，功能神经影像技术在癫痫的临床应用 由于其时间和空间分辨率不令人满意， 内源性信号光学成像是一种功能性神经成像技术 一种测量皮层反射率变化的技术， 分辨率和微米空间分辨率。这些光学图像相互关联 与神经元活动，并由于脑血容量的变化，光 散射，以及血红蛋白和细胞色素氧化态。在 初步研究中，OIS记录自大鼠的躯体感觉皮层， 近红外频率（850 nm）。OIS显示与癫痫发作密切相关 活动，以可恢复和可复制的方式。还注意到 OIS变化可能先于初始EEG尖峰长达1分钟。目标 这个项目的目的是将多波长光信号与 电生理学和免疫组织化学标记，并建立一个 解释OIS缉获数据的主要基础。的结果 该项目可以在未来应用于各种癫痫模型，如在一个 皮层点燃模型用于研究神经元网络行为 点燃过程OIS成像可能具有更好的潜力， 了解癫痫发生、癫痫发作诱导、停止和传播 途径。OIS成像在癫痫手术中的额外应用 明确功能皮层并确定癫痫病灶， 在未来彻底探索。候选人陈医生是一名神经科医生 以及一位细胞电生理学家，他擅长使用膜片钳技术 法他的职业目标是阐明癫痫的基本机制， 整合他已经学到或将要学到的所有研究技能， 电生理学、光学成像、神经化学和计算机模拟。 该项目将在加州大学洛杉矶分校医学院进行，由Toga博士领导 和韦斯特兰的指引两位导师都是国际知名的权威人士 在各自的研究领域。

DESCRIPTION (provided by applicant): The development of both structural and functional neuroimaging has progressed significantly in the last decade. However, clinical application of functional neuroimaging techniques in epilepsy has been complicated by their unsatisfactory temporal and spatial resolution. Optical imaging of intrinsic signals (OIS) is a functional neuroimaging technique that measures cortical reflectance changes with millisecond temporal resolution and micron spatial resolution. These optical images are correlated with neuronal activity and are due to changes in cerebral blood volume, light scattering, and in hemoglobin and cytochrome oxidation state. In the preliminary studies, OIS was recorded from the somatosensory cortex of rats at, near infrared frequency (850 nm). OIS was shown to correlate well with seizure activities, in a recoverable and reproducible fashion. It was also noted that OIS changes could precede the initial EEG spikes for up to I minute. The goal of this project is to correlate multiplewavelength optical signals with both electrophysiological and immunohistochemical markers, and to establish a primary foundation of interpreting OIS seizure data. The results of this project can be applied in the future in various epilepsy models, such as in a cortical kindling model for the study of neuronal network behaviors during kindling process. OIS imaging might hold a promising potential of better understanding of epileptogenesis, seizure induction, cessation and propagation pathways. Additional application of OIS imaging during epilepsy surgery in defining the eloquent cortex and confirming the seizure focus should be explored thoroughly in the future. The candidate, Dr. Chen, is a neurologist and a cellular electrophysiologist who has the expertise of using patch clamp technique. His career goal is to elucidate the basic mechanisms of epilepsy by integrating all the research skills that he has learned or will learn in electrophysiology, optical imaging, neurochemistry and computer simulation. This project will be conducted at the UCLA School of Medicine under Drs. Toga and Wasterlain's guidance. Both mentors are internationally known authorities in their respective fields of research.