Optical Intrinsic Signal Imaging of Seizure

癫痫发作的光学本征信号成像

• 批准号: 6620682
• 负责人: JAMES WY CHEN
• 金额: $ 16.14万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6620682
• 关键词: blood volume; brain circulation; brain electrical activity; brain imaging /visualization /scanning; brain mapping; charge coupled device camera; computer simulation; cytochromes; disease /disorder model; electrodes; electroencephalography; electrophysiology; epilepsy; hemoglobin; immunocytochemistry; kindling; laboratory rat; light scattering; neurochemistry; neuroimaging; neurons; optics; oxidation; pathologic process; prognosis; temporal lobe /cortex

DESCRIPTION (provided by applicant): The development of both structural and functional neuroimaging has progressed significantly in the last decade. However, clinical application of functional neuroimaging techniques in epilepsy has been complicated by their unsatisfactory temporal and spatial resolution. Optical imaging of intrinsic signals (OIS) is a functional neuroimaging technique that measures cortical reflectance changes with millisecond temporal resolution and micron spatial resolution. These optical images are correlated with neuronal activity and are due to changes in cerebral blood volume, light scattering, and in hemoglobin and cytochrome oxidation state. In the preliminary studies, OIS was recorded from the somatosensory cortex of rats at, near infrared frequency (850 nm). OIS was shown to correlate well with seizure activities, in a recoverable and reproducible fashion. It was also noted that OIS changes could precede the initial EEG spikes for up to I minute. The goal of this project is to correlate multiplewavelength optical signals with both electrophysiological and immunohistochemical markers, and to establish a primary foundation of interpreting OIS seizure data. The results of this project can be applied in the future in various epilepsy models, such as in a cortical kindling model for the study of neuronal network behaviors during kindling process. OIS imaging might hold a promising potential of better understanding of epileptogenesis, seizure induction, cessation and propagation pathways. Additional application of OIS imaging during epilepsy surgery in defining the eloquent cortex and confirming the seizure focus should be explored thoroughly in the future. The candidate, Dr. Chen, is a neurologist and a cellular electrophysiologist who has the expertise of using patch clamp technique. His career goal is to elucidate the basic mechanisms of epilepsy by integrating all the research skills that he has learned or will learn in electrophysiology, optical imaging, neurochemistry and computer simulation. This project will be conducted at the UCLA School of Medicine under Drs. Toga and Wasterlain's guidance. Both mentors are internationally known authorities in their respective fields of research.

描述（由申请人提供）：结构和技术的发展 功能神经影像学在过去十年中取得了显着进展。 然而，功能性神经影像技术在癫痫中的临床应用 由于其不令人满意的时间和空间分辨率而变得复杂。 内在信号光学成像（OIS）是一种功能性神经成像 测量毫秒时间皮质反射率变化的技术 分辨率和微米空间分辨率。这些光学图像是相关的 与神经元活动有关，并且是由于脑血容量的变化、光 散射，并处于血红蛋白和细胞色素氧化状态。在 初步研究，OIS 是从大鼠体感皮层记录的， 近红外频率（850 nm）。 OIS 被证明与癫痫发作密切相关 以可恢复和可重复的方式进行活动。还注意到 OIS 变化可能会先于最初的 EEG 峰值长达 1 分钟。目标 该项目的目的是将多波长光信号与 电生理学和免疫组织化学标记物，并建立 解释 OIS 癫痫数据的主要基础。这样做的结果 该项目将来可以应用于各种癫痫模型，例如 用于研究神经元网络行为的皮层点燃模型 点燃过程。 OIS 成像可能具有更好的前景 了解癫痫发生、癫痫发作诱发、停止和蔓延 途径。 OIS 成像在癫痫手术中的附加应用 定义雄辩皮层并确认癫痫病灶应该 未来要好好探索。候选人陈博士是一位神经科医生 以及一位具有使用膜片钳专业知识的细胞电生理学家 技术。他的职业目标是通过以下方式阐明癫痫的基本机制： 整合他已经学到或将要学到的所有研究技能 电生理学、光学成像、神经化学和计算机模拟。 该项目将在加州大学洛杉矶分校医学院由博士进行。长袍 和沃斯特莱恩的指导。两位导师均为国际知名权威 在各自的研究领域。