Development and application of automated multiwell plate fluorescence microscopy

自动化多孔板荧光显微镜的研制与应用

• 批准号: 2123641
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2123641
• 关键词: Development; application; automated; multiwell; plate

This project involves the development of a modular open source automated fluorescence microscope platform based on Micromanager and incorporating an optical autofocus module. This will enable automated fluorescence lifetime imaging (FLIM) for assays of protein interactions and changes in cell metabolic processes. A multibeam multiphoton excitation scheme will be explored for optical sectioning and label-free contrast based on autofluorescence. Automated super-resolved imaging with single molecule localisation microscopy techniques will also be implemented. This platform will be developed with open source software and low-cost components where appropriate to widen access to these advanced microscopy techniques. Applications of these imaging modalities to medical research will be explored including to heterogeneity in the host-pathogen of macrophages for COPD, to studies of cellular metabolic changes associated with the differentiation of embryonic stem cells and with cancer and to the temporal and spatial regulation of activation-induced deoxycytidine deaminase (AID) in the cell nucleus.

该项目涉及基于Micromanager的模块化开源自动荧光显微镜平台的开发，并包含光学自动对焦模块。这将使自动荧光寿命成像（FLIM）用于蛋白质相互作用和细胞代谢过程变化的测定。一个多光束多光子激发计划将探索光学切片和标记的基础上自发荧光的对比。还将采用单分子定位显微镜技术进行自动超分辨成像。该平台将使用开源软件和低成本组件开发，以扩大对这些先进显微技术的访问。这些成像模式的医学研究的应用将探讨包括异质性的宿主-病原体的巨噬细胞的COPD，与胚胎干细胞的分化和癌症的细胞代谢变化的研究和激活诱导的脱氧胞苷脱氨酶（AID）在细胞核中的时间和空间调节。