Near IR fluorescent probes/molecular analysis of cancer

近红外荧光探针/癌症分子分析

• 批准号: 6608548
• 负责人: Alan S. Waggoner
• 金额: $ 50.08万
• 依托单位: CARNEGIE-MELLON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-07-18 至 2005-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6608548
• 关键词: anthracenes; chemical stability; chemical structure function; chemical synthesis; chromophore; cyanine; cyclodextrins; flow cytometry; fluorescent dye /probe; imaging /visualization /scanning; infrared radiation; microcapsule; microscopy; neoplasm /cancer diagnosis; nucleic acids; technology /technique development; tissue /cell culture

DESCRIPTION (provided by applicant): It is well known that fluorescence detection provides the basis of flow cytometry, fluorescence imaging, DNA sequencing, microarray detection, drug testing, diagnostics, and so on. It is our goal to extend the power of fluorescence detection in cancer research and diagnostics, by developing new fluorescent probes that absorb and fluoresce in the red and near infrared regions of the spectrum. These advances will: (1) increase the number of parameters that can be acquired in a single experiment (hence, more information becomes available through correlation of cell/tissue properties), (2) increase sensitivity (since cellular autofluorescence is much smaller in the red and near IR), and (3) allow imaging deeper into tissues (since absorbance and fluorescence will be away from hemoglobin absorption and in a range where light scattering and absorption is reduced). The new fluorescent reagents will be photostable, chemically stable, water soluble, brightly fluorescent and non-photo-toxic to cells and tissues. This will be accomplished by synthetic modifications in the molecular structure of cyanine (polymethine) dyes and by stabilizing dye structure through encapsulation methods including cyanine-cyclodextrin rotaxanes, nucleic acid encapsulation, sandwich complexation, and covalent self-encapsulation.

描述（由申请人提供）： 众所周知，荧光检测提供了流动的基础 流式细胞术，荧光成像，DNA测序，微阵列检测，药物 测试、诊断等。我们的目标是扩展 荧光检测在癌症研究和诊断，通过开发新的 在红色和近红外吸收并发出荧光的荧光探针 光谱的区域。这些进展将：（1）增加 可以在单个实验中获得的参数（因此，更多 信息通过细胞/组织特性的相关性变得可用）， (2)增加灵敏度（因为细胞自发荧光在 红色和近红外），以及（3）允许成像更深入组织（因为 吸光度和荧光将远离血红蛋白吸收， 光散射和吸收减少的范围）。的新的荧光 试剂将是光稳定的、化学稳定的、水溶性的、明亮的、 对细胞和组织具有荧光性和非光毒性。这将是 通过对花青的分子结构进行合成修饰来完成 （聚甲炔）染料和通过包封稳定染料结构 方法包括花青-环糊精轮烷，核酸包封， 夹心络合和共价自包封。