Systematic Vertebrate Functional Genomics

系统脊椎动物功能基因组学

• 批准号: 6798021
• 负责人: Stephen Carl Ekker
• 金额: $ 3万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-01 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6798021
• 关键词: biological signal transduction; cell cell interaction; developmental genetics; embryogenesis; fluorescence microscopy; functional /structural genomics; gene expression; genetic screening; growth /development; histogenesis; in situ hybridization; molecular biology information system; neurogenesis; organ; protein structure function; secretory protein; thin layer chromatography; transcription factor; vertebrate embryology; zebrafish

DESCRIPTION (provided by applicant): The vertebrate genome contains a predicted 100,000 genes, most of unknown function. The recent development of morpholino-based gene knockdown technology in zebrafish has opened the door to the genome-wide assignment of function based on sequence in a model vertebrate. We propose to explore the molecular mechanisms that control fundamental vertebrate embryonic processes of patterning and organ formation, biological problems in which cell-cell communication is critical during development. We will focus this study on the systematic assignment of biological function to a set of 100-200 putative secreted proteins to identify key players in these intercellular embryonic signaling cascades. We will accomplish this goal through the following specific aims: Aim I. Development of a secreted protein morpholino core database. Genes encoding predicted secreted proteins will be selected through analysis of the zebrafish genomic and EST database. Morpholinos targeted to these genes will be generated and examined for function in the following biological processes: Aim II. Isolation of secreted proteins required for early embryonic and neural patterning. Aim III. Isolation of secreted proteins required for cardiovascular and sensory organ formation. Aim IV. Isolation of secreted proteins required for digestive organ formation and function. The identification of molecules required for vertebrate patterning and organ formation has critical implications for the understanding of genetic deficiencies in these processes. In addition, these signaling proteins, crucial for development in vivo, will be crucial to the establishment of conditions for in vitro organ formation. The zebrafish offers the first comprehensive analysis of these processes using as template an entire vertebrate genome.

描述（由申请人提供）：脊椎动物基因组预计包含100,000个基因，大部分功能未知。的最新发展