MICROBIAL PATHOGENS IN RODENT GAMETE AND EMBRYO BANKING

啮齿动物配子和胚胎库中的微生物病原体

• 批准号: 6663820
• 负责人: LELA K. RILEY
• 金额: $ 23.79万
• 依托单位: UNIVERSITY OF MISSOURI-COLUMBIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-09-30 至 2006-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6663820
• 关键词: Rodentias; antiinfective agents; cryopreservation; cryoprotective agents; disease /disorder model; embryo /fetus preservation; environmental contamination; germ cells; laboratory mouse; laboratory rat; method development; microorganism classification; microorganism disease chemotherapy; microorganism growth; polymerase chain reaction; tissue resource /registry; vertical transmission; virulence

DESCRIPTION (Provided by applicant): With the pending completion of the Human Genome Project, scientists will soon know the sequence of all human genes. This knowledge will create an unprecedented need for new rodent models to identify and understand gene function to test new therapeutic strategies aimed at alleviating human diseases. Maintenance of the exponentially increasing numbers of these mutant strains as live animals will not be feasible. Cryopreservation and genome banking of spermatozoa and oocytes (gametes) and embryos will be mandatory. However, there are two major impediments to genome banking of rodent models. Many mutant mouse and rat lines are infected with microbial pathogens. An understanding of the risks of transmission of infectious agents through gametes and embryos must be developed. Preservation of gametes and embryos will be compromised if they are contaminated with agents that lead to infection in the regenerated mutant lines and cause outbreaks of infectious disease. Methods to efficiently freeze mouse embryos and regenerate strains stocks from frozen stocks via embryo transfer are well established for the mouse, but little work has been done in developing efficient rat embryo cryopreservation methodology. The Specific Aims of this application are to: 1) determine which of the commonly found murine pathogens contaminate mouse and rat gametes and embryos; 2) develop methods to remove microbial pathogens from rodent gametes and embryos and thus, decrease the likelihood of their transmission during genome banking; and 3) develop improved rat embryo cryopreservation methods.

描述（由申请人提供）：随着人类基因组计划的即将完成，科学家将很快知道所有人类基因的序列。