REMINERALIZING PULP-CAPPING CEMENTS & BONDING AGENTS

该研究的长期目标是证明先前开发的Ca- po4树脂水泥（CP-RC）的静牙性能和生物相容性，并研究一种新型Ca- po4富集粘合剂（CP-BA）的体外和体内性能。CP-RC和CP-BA都是基于酸性和其他二甲基丙烯酸酯树脂，Ca-PO4填料和氟添加剂。该水泥将提供其作为直接或间接牙髓封盖剂的临床使用所必需的处理特性。它在固化期间和固化后具有较高的pH值，适度的氟化物释放，并已证明在体外对人工牙病变再矿化。然而，尽管该水泥具有中等的粘结性能，但与现有的粘结剂相比，其与牙本质的粘结强度较低。此外，初步试验表明，在CP-RC与人工龋齿样病变的牙本质之间放置粘结剂可显著减少其再矿化。因此，有必要探索具有再矿化/固牙性能的新型牙本质粘结剂，并对正常牙本质和缺乏矿物质的牙本质具有很强的粘结力。这种胶粘剂可以作为传统的恢复性粘接剂和纸浆封盖用途。具体目标是：1。评估CP-RC预防邻近牙本质和牙釉质继发性龋形成的有效性；2. (i)从水泥中定性和定量地分析可浸出成分；（ii）确定体外细胞毒性和对成牙细胞培养的影响，并将CP-RC的体外生物相容性和细胞分化与正在进行的水泥盖髓效果的体内动物研究结果进行比较；3. 研究含有与CP-RC相同的粘合树脂和Ca-PO4填料的粘合体系，并表征其生物活性效应和强化机制4。评价优化后的CP-BA和5的生物相容性和细胞分化。通过评估这种新型生物相容性、生物活性结合剂的体内盖髓效果，解决粘接剂能否成功应用于盖髓程序的争议性问题。这项研究的完成将导致再矿化牙髓覆盖/内衬水泥和生物活性Ca-PO4牙本质粘合剂。CaPO4树脂水泥将提供一种可靠的方法，通过刺激牙本质桥接的自然形成，使其具有适度的粘连性，以防止泄漏，并具有立即放置永久修复的强度，从而可靠地治疗机械和小蛀牙牙髓暴露。再矿化胶粘剂将提供粘接缺乏矿物质的牙本质和覆盖暴露的牙髓的方法。拟议的研究还将提供有价值的体内试验结果与牙髓细胞体外反应的比较。

The long-term goals of the proposed research are to demonstrate the cariostatic properties and biocompatibility of a previously developed Ca-PO4 Resin Cement (CP-RC) and to investigate the in vitro and in vivo properties of a novel Ca- PO4-enriched Bonding Agent (CP-BA). Both CP-RC and CP-BA are based on acidic- and other dimethacrylate resins, Ca-PO4 fillers and fluoride additives. The cement will provide the handling characteristics necessary for its clinical use as a direct or indirect pulp-capping agent. It has high pH during and after setting, moderate fluoride release, and has been shown in vitro to remineralize artificial tooth lesions. However, while the cement has shown moderate adhesive properties, its bond strength to dentin is low compared to those of current bonding agents. Moreover, preliminary tests showed that bonding agents placed between CP-RC and dentin with artificial caries-like lesions significantly reduced their remineralization. Thus, there is a need to explore novel dentin bonding agents with remineralizing/cariostatic properties and strong adhesion to normal and mineral-deficient dentin. Such adhesives could serve as conventional restorative bonding agents and for pulp-capping purposes. The specific aims are to 1. evaluate CP-RC effectiveness for preventing secondary caries formation in adjacent dentin and enamel; 2. (i) analyze qualitatively and quantitatively leachable components from the cement; and (ii) determine the in vitro cytotoxicity and effects on odontoblast cell culture and compare the in vitro biocompatibility and cytodifferentiation of CP-RC with the results from an (ongoing) in vivo animal study on the pulp-capping efficacy of the cement; 3. investigate bonding systems containing the same adhesive resin and Ca-PO4 fillers as the CP-RC and characterize their bioactivity effects and reinforcing mechanisms 4. evaluate the biocompatibility and cytodifferentiation of an optimized CP-BA and 5. address the controversial question of whether adhesive bonding agents can be successfully applied to pulp-capping procedures by evaluating the in vivo pulp-capping efficacy of this novel biocompatible, bioactive bonding agent. Completion of this research will lead to a remineralizing pulp-capping/lining cement and a bioactive Ca-PO4 dentin adhesive The CaPO4 resin cement will provide a method to reliably treat mechanical and small carious pulp exposures by stimulating the natural formation of dentin bridging with a base that has moderate adhesion to prevent leakage and the strength to allow the immediate placement of a permanent restoration. A remineralizing adhesive will provide methods of adhering to mineral-deficient dentin and for covering exposed pulps. The proposed studies will also provide a valuable comparison of in vivo test results to pulp cell response in vitro.