REMINERALIZING PULP-CAPPING CEMENTS & BONDING AGENTS

再矿化盖浆水泥

• 批准号: 6382541
• 负责人: SABINE H DICKENS
• 金额: $ 26.14万
• 依托单位: AMERICAN DENTAL ASSOCIATION FOUNDATION
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-09-15 至 2004-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6382541
• 关键词: biomaterial compatibility; biomaterial evaluation; calcium phosphate; cell adhesion; cell differentiation; cell proliferation; cementum; composite resins; cytotoxicity; dental adhesive /sealant; dental bonding material; dental caries; dentin; disease /disorder prevention /control; dogs; normal ossification; nuclear magnetic resonance spectroscopy; odontoblasts; oral facial restoration; polymerase chain reaction; polymethacrylate; root canal therapy; tissue /cell culture; tooth enamel

The long-term goals of the proposed research are to demonstrate the cariostatic properties and biocompatibility of a previously developed Ca-PO4 Resin Cement (CP-RC) and to investigate the in vitro and in vivo properties of a novel Ca- PO4-enriched Bonding Agent (CP-BA). Both CP-RC and CP-BA are based on acidic- and other dimethacrylate resins, Ca-PO4 fillers and fluoride additives. The cement will provide the handling characteristics necessary for its clinical use as a direct or indirect pulp-capping agent. It has high pH during and after setting, moderate fluoride release, and has been shown in vitro to remineralize artificial tooth lesions. However, while the cement has shown moderate adhesive properties, its bond strength to dentin is low compared to those of current bonding agents. Moreover, preliminary tests showed that bonding agents placed between CP-RC and dentin with artificial caries-like lesions significantly reduced their remineralization. Thus, there is a need to explore novel dentin bonding agents with remineralizing/cariostatic properties and strong adhesion to normal and mineral-deficient dentin. Such adhesives could serve as conventional restorative bonding agents and for pulp-capping purposes. The specific aims are to 1. evaluate CP-RC effectiveness for preventing secondary caries formation in adjacent dentin and enamel; 2. (i) analyze qualitatively and quantitatively leachable components from the cement; and (ii) determine the in vitro cytotoxicity and effects on odontoblast cell culture and compare the in vitro biocompatibility and cytodifferentiation of CP-RC with the results from an (ongoing) in vivo animal study on the pulp-capping efficacy of the cement; 3. investigate bonding systems containing the same adhesive resin and Ca-PO4 fillers as the CP-RC and characterize their bioactivity effects and reinforcing mechanisms 4. evaluate the biocompatibility and cytodifferentiation of an optimized CP-BA and 5. address the controversial question of whether adhesive bonding agents can be successfully applied to pulp-capping procedures by evaluating the in vivo pulp-capping efficacy of this novel biocompatible, bioactive bonding agent. Completion of this research will lead to a remineralizing pulp-capping/lining cement and a bioactive Ca-PO4 dentin adhesive The CaPO4 resin cement will provide a method to reliably treat mechanical and small carious pulp exposures by stimulating the natural formation of dentin bridging with a base that has moderate adhesion to prevent leakage and the strength to allow the immediate placement of a permanent restoration. A remineralizing adhesive will provide methods of adhering to mineral-deficient dentin and for covering exposed pulps. The proposed studies will also provide a valuable comparison of in vivo test results to pulp cell response in vitro.

这项研究的长期目标是证明先前开发的钙-磷酸树脂粘固剂(CP-RC)的防龋性和生物相容性，并研究新型富钙粘结剂(CP-BA)的体外和体内性能。CP-RC和CP-BA都是基于酸性和其他二甲基丙烯酸酯树脂、Ca-PO4填料和氟化物添加剂。该粘固剂将提供作为直接或间接盖髓剂的临床使用所需的操作特性。它在固化过程中和固化后具有较高的pH值，适中的氟化物释放，并已在体外被证明可使人工牙齿损伤重新矿化。然而，虽然该粘结剂具有中等的粘接性能，但与当前粘结剂相比，其与牙本质的粘结强度较低。此外，初步测试表明，放置在CP-RC和具有人工龋样病变的牙本质之间的粘结剂显著减少了它们的再矿化。因此，有必要探索新的牙本质粘结剂，该粘结剂具有再矿化/防龋性，并且对正常和矿物质缺乏的牙本质具有很强的粘附性。这种粘合剂可用作传统的修复性粘结剂，也可用于盖髓。其具体目的是：1.评价CP-RC预防邻牙本质和牙釉质继发性龋病形成的有效性；2.定性和定量分析粘固剂中的可溶出成分；以及(Ii)测定体外细胞毒性和对成牙本质细胞培养的影响，并将CP-RC的体外生物相容性和细胞分化与正在进行的体内动物实验结果进行比较。3.研究与CP-RC相同的粘接树脂和Ca-PO4填料的粘接体系，并表征其生物活性效应和增强机制4.评价优化后的CP-BA的生物相容性和细胞分化；5.通过评价这种新型生物相容性、生物活性粘结剂的体内盖髓效果，解决粘结剂能否成功应用于盖髓过程的争议问题。这项研究的完成将导致一种再矿化的牙髓盖盖/衬里粘固剂和一种生物活性的Ca-PO4牙本质粘结剂。CaPO4树脂粘固剂将提供一种可靠的方法，通过刺激牙本质与具有中等粘附性的基托的自然形成来桥接牙本质，防止渗漏，并提供允许立即放置永久修复体的强度，从而可靠地治疗机械性和小型龋齿牙髓暴露。再矿化粘合剂将提供粘接矿物质缺乏的牙本质和覆盖暴露的牙髓的方法。拟议的研究还将提供体内测试结果与体外牙髓细胞反应的有价值的比较。