OPTICAL BIOPSY USING OPTICAL COHERENCE TOMOGRAPHY

使用光学相干断层扫描进行光学活检

• 批准号: 6647187
• 负责人: JAMES G FUJIMOTO
• 金额: $ 25.59万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2004-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6647187
• 关键词: bioimaging /biomedical imaging; biomedical equipment development; clinical research; diagnosis design /evaluation; disease /disorder model; early diagnosis; fiber optic microscopy; hamsters; human subject; imaging /visualization /scanning; neoplasm /cancer diagnosis; tomography

DESCRIPTION (Adapted from Applicant's Abstract): This is a multidisciplinary collaborative program involving researchers at the Massachusetts Institute of Technology, Harvard Medical School, Massachusetts General Hospital, Brigham and Women's Hospital, and the National Cancer Institute. The hypothesis of this proposal is that optical coherence tomography (OCT), an emerging biomedical diagnostic imaging technology for in situ imaging of tissue microstructure, can be developed and applied for "optical biopsy," the real time, in vivo differentiation and monitoring of early neoplastic changes. This program integrates the development of new technology with its application to fundamental and clinical studies in oncology. The applicants propose to: 1. Develop ultrahigh resolution, spectroscopic, and Doppler OCT technology which extends the current 10-15 (m image resolution to the 1-2 (m level. This is a quantum leap in performance which will significantly improve imaging of architectural morphology and facilitate imaging of cellular features for identifying early neoplastic changes. Spectroscopic and Doppler OCT could enable functional imaging of tissue on a micron scale. 2. Develop delivery instruments for OCT which allow noninvasive or minimally invasive clinical imaging. These include a catheter/endoscope, colposcope, hysteroscope, hand held probe, and needle. 3. Investigate OCT imaging of early neoplastic changes and cancer progression in an animal model to define markers of dysplasia and to develop quantitative assessments of vascular density. A noninvasive, real time technique for quantitating angiogenesis can be a powerful research tool for the development of anti-angiogenesis agents. 4. Investigate OCT imaging in the female reproductive tract including the cervix and endometrium. The cervix is an excellent in vivo model system to establish markers of dysplastic change. Hysteroscopic OCT imaging of the uterus could improve the detection of endometrial dysplasia and cancer. 5. Investigate OCT imaging of dysplasia and leukoplakia of oral mucosa and chemoprevention treatment. Approaches to quantitatively assess response to chemoprevention would be important tools for pharmacological trials. 6. Investigate OCT imaging of the GI tract. Explore the feasibility of OCT for differentiating dysplasia and adenocarcinoma of the esophagus to guide conventional excisional biopsy and reduce false negative rates. Develop OCT as a low cost screening technique for Barrett's esophagus. Taken together, these studies will provide new imaging tools for oncology research and also develop new clinical diagnostic and screening techniques.

描述（改编自申请人的摘要）：这是一个多学科的 马萨诸塞州研究所的研究人员参与了一项合作计划， 技术、哈佛医学院、马萨诸塞州总医院、布里格姆和 妇女医院和国家癌症研究所。这个假设 一种新兴的生物医学成像技术--光学相干断层扫描（OCT） 用于组织微结构的原位成像的诊断成像技术，可以 开发和应用“光学活检”，真实的时间，在体内 分化和监测早期肿瘤变化。这个程序 将新技术的开发与应用结合起来， 肿瘤学的基础和临床研究。申请人建议：1. 开发高分辨率、光谱和多普勒OCT技术， 将当前10-15 μ m的图像分辨率扩展到1-2 μ m的水平。这是一 性能上的巨大飞跃，将显著改善 结构形态学和促进细胞特征的成像， 识别早期肿瘤变化。光谱和多普勒OCT可以 能够在微米尺度上对组织进行功能成像。2.开发交付 用于OCT的仪器，允许无创或微创临床 显像这些包括导管/内窥镜、阴道镜、宫腔镜、手 手持探针和针。3.研究早期肿瘤变化的OCT成像 和癌症进展，以确定发育异常的标志物， 发展血管密度的定量评估。一个非侵入性的，真实的时间 定量血管生成的技术可以是一个强大的研究工具， 开发抗血管生成剂。4.研究OCT成像， 女性生殖道，包括子宫颈和子宫内膜。子宫颈 一个极好的体内模型系统，以建立发育异常变化的标志物。 子宫镜OCT成像可以提高子宫内膜异位症的检出率。 子宫内膜发育不良和癌症。5.研究发育不良的OCT成像， 口腔粘膜白斑及化学预防治疗。方法来 定量评估对化学预防的反应将是 药理学试验6.研究胃肠道的OCT成像。探索 光学相干断层扫描（OCT）用于鉴别非典型增生和腺癌的可行性 食管引导常规切除活检减少假阴性 rates.开发OCT作为Barrett食管的低成本筛查技术。 总而言之，这些研究将为肿瘤学提供新的成像工具 研究并开发新的临床诊断和筛查技术。