Investigations of the link between the bacterial cytoskeleton and N-cycling in the Rhizobium-legume symbiosis

根瘤菌-豆科植物共生中细菌细胞骨架与氮循环之间联系的研究

• 批准号: 2246559
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2246559
• 关键词: Investigations; link; between; bacterial; cytoskeleton

Nitrogen fixation is the conversion of atmospheric nitrogen into ammonia and is a part of biogeochemical nitrogen cycling. Rhizobium bacteria are found in soil and can form a symbiosis with the roots of legume plants, where the bacteria fix nitrogen and provide a direct source of ammonia to the legume. The Rhizobium-legume symbiosis has significant impact on agricultural practices greatly reducing pollution generated by the use of nitrogen fertilisers. Although bacterial growth has been studied for many years, very little is known about the mechanism of growth during the entry of Rhizobium bacteria into their cognate pea-plant. We will study the poorly understood mechanism of polar growth and its link to N-cycling amongst Rhizobiales and monitor the diversity of polar protein assemblies by analysing soil samples from crop-fields at several UK locations. In collaboration with PGRO (Processors and Growers Research Organisation) the bacterial samples will be collected and analysed. Microbial molecular biology technologies including the generation of knockout mutations and fluorescent protein fusions will be used to study the polar protein assemblies. Finally, mutants will be tested for their ability to establish successful symbiosis with pea plants.

固氮是将大气中的氮转化为氨，是生物地球化学氮循环的一部分。根瘤菌存在于土壤中，可以与豆科植物的根形成共生关系，在那里细菌固定氮并为豆科植物提供氨的直接来源。根瘤菌与豆科植物的共生关系对农业实践具有重要影响，大大减少了氮肥使用所产生的污染。虽然对细菌的生长进行了多年的研究，但对根瘤菌进入其同源植物豌豆的生长机制知之甚少。我们将研究极地生长的机制及其与根瘤菌间n循环的联系，并通过分析英国几个地区农田的土壤样本来监测极地蛋白质组合的多样性。与PGRO（加工者和种植者研究组织）合作，将收集和分析细菌样本。微生物分子生物学技术，包括产生敲除突变和荧光蛋白融合将用于研究极性蛋白组装。最后，将测试突变体与豌豆植物成功建立共生关系的能力。