Gas Chromatograph Mass Spectrometer with Combined FID

结合 FID 的气相色谱质谱仪

• 批准号: 6440774
• 负责人: Elena Volpi
• 金额: $ 14.59万
• 依托单位: UNIVERSITY OF SOUTHERN CALIFORNIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6440774
• 关键词: biomedical equipment purchase; gas chromatography mass spectrometry; glucose metabolism; ionization; lipid metabolism; stable isotope

DESCRIPTION (provided by applicant): We are requesting a gas chromatograph (GC) mass spectrometer (MS) with quadrupole detector, and flame ionization detector (FID) attached to a second column lodged in the same GC. The system will be completed by a speed-vacuum system for sample preparation. This system will be used for analysis of samples deriving from metabolic studies performed in vivo in humans and animals using stable isotope tracers. Specifically, the GCMS will be mostly used for the determination of the isotopic enrichment of glucose in blood and tissue samples, although we do not exclude that we will use also for glycerol, fatty acid, and amino acid analysis. The expected enrichments (3-10 percent) will be in the optimal detection range of the instrument, which is 0.1-100 percent. The FID will be used for analysis of lipid concentrations and composition pattern in biological samples, such as blood and tissue. The MS detector and the FID can potentially be used in combination if the GC oven program is the same for both type of analysis. The GCMS with FID system will allow us to analyze our samples with a better quality control, faster sample turnover and more direct interaction with the laboratory that measures our samples. This will in turn improve the quality of our research, and reduce the analytical time.

描述（由申请人提供）：我们要求使用气相色谱仪（GC） 质谱仪（MS），带四极杆检测器和火焰电离检测器 （FID）连接到同一GC中的第二列。系统将是 由用于样品制备的速度效率系统完成。这个系统将是 用于分析来自体内代谢研究的样品 在人类和动物中，使用稳定的同位素示踪剂。 具体而言，GCMS主要用于确定 同位素在血液和组织样品中的同位素富集，尽管我们不 排除我们还将用于甘油，脂肪酸和氨基酸 分析。预期的富集（3-10％）将是最佳的 仪器的检测范围为0.1-100％。 FID将是 用于分析生物学中脂质浓度和组成模式 样品，例如血液和组织。 MS检测器和FID可能有可能 如果两种类型的GC烤箱程序相同，则可以组合使用 分析。 具有FID系统的GCM将使我们能够更好地分析样品 质量控制，更快的样本周转率以及与 测量我们样本的实验室。反过来将提高 我们的研究，并减少分析时间。