BSL3 Ultra High Speed Flow Cytometer

描述（由申请人提供）：临床研究人员和基础科学家 在费城儿童医院和大学校园里 包括宾夕法尼亚大学医院， 宾夕法尼亚大学医学院，以及附近的 越来越多的研究机构参与研究项目， 使用高度复杂的流式细胞术和细胞分选仪器。 这项技术对许多资助项目的成功尤为关键 宾夕法尼亚大学癌症中心的研究人员 和艾滋病研究中心对于这些调查人员来说， 潜在致病物质，例如艾滋病毒、肝炎、单纯疱疹等 其他人，获得流式细胞术和细胞分选活的，未固定的材料， 目前在费城地区还没有。因为潜在的 由于这些制剂的生物危害性，不可能进行以下研究 在常规的流式细胞术设备中检测含有活性剂的细胞。这 这对那些从事研究的研究人员来说是一个严重的限制。 细胞功能 BSL 3流式细胞术和细胞分选设施将建立在目前的 成功的集中式流式细胞仪服务可在大学 宾夕法尼亚州校园提供多样化，技术先进的细胞分选 和分析服务，为那些调查人员工作， 生物危害材料。这些服务对于了解基本的 细胞活化和分化的过程，以及寻找 这些过程与多种病症如AIDS的翻译相关性， 肺结核、肝炎和肿瘤。作为集中 资源，BSL 3设施不仅将提供仪器和服务 但将与调查人员密切合作， 实验设计、数据分析和数据解释。基于高度 宾夕法尼亚大学癌症中心的成功经验 细胞计数设施，预计这种密切的关系将刺激 促进研究人员之间的互动 参与研究。用户研究兴趣的范围反映在 下面介绍的各种项目。用户需求的多样性 需要维护高度通用的、最先进的BSL 3流 细胞仪设施配备了科学家和技术人员， 专业知识Cytomation MoFlo超高速流式细胞仪提供了 在BSL-3环境中进行分拣的最佳平台。

DESCRIPTION (provided by applicant): Clinical researchers and basic scientists at the Children's Hospital of Philadelphia and on the campus of the University of Pennsylvania, including the Hospital of the University of Pennsylvania and the University of Pennsylvania School of Medicine, as well as those at nearby institutions, are increasingly engaged in research projects that require the use of highly sophisticated flow cytometry and cell sorting instrumentation. This technology is particularly critical to the success of many funded projects engaged in by investigators from the University of Pennsylvania Cancer Center and the Center for AIDS Research. For those investigators, working with potentially pathogenic materials, such as HIV, hepatitis, herpes simplex, among others, access to flow cytometry and cell sorting for live, unfixed material is not currently available in the Philadelphia area. Because of the potentially biohazardous nature of these agents, it is not possible to perform studies of cells containing live agents in a conventional flow cytometry facility. This represents a serious limitation for those investigators engaged in research on cell function. The BSL3 Flow Cytometry and Cell Sorting Facility will build on the currently successful centralized flow cytometry services available on the University of Pennsylvania campus to provide diverse, technically sophisticated cell sorting and analysis services for those investigators working with potentially biohazardous materials. These services are critical to understanding the basic processes of cell activation and differentiation as well as to finding the translational relevance of such processes to multiple conditions such as AIDS, tuberculosis, hepatitis, and the neoplastic condition. As a centralized resource, the BSL3 facility will not only provide instrumentation and services in a cost-effect manner, but will work closely with investigators on experimental design, data analysis and data interpretation. Based on the highly successful experience of the University of Pennsylvania Cancer Center Flow Cytometry Facility, it is expected that this close relationship will stimulate new applications by researchers and facilitate interactions among investigators involved in research. The range of user research interests is reflected in the diversity of projects presented below. This diversity of user needs necessitates the maintenance of a highly versatile, state-of-the-art BSL3 flow cytometry facility staffed by scientists and technicians with recognized expertise. The Cytomation MoFlo Ultra-High Speed Flow Cytometer provides an optimal platform for sorting in a BSL-3 setting.