Hybrid Quadrupole-Time of Flight Mass Spectrometer

混合四极杆飞行时间质谱仪

• 批准号: 6441335
• 负责人: WILLIAM M PIERCE
• 金额: $ 50万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6441335
• 关键词: biomedical equipment purchase; chemical structure; intermolecular interaction; mass spectrometry

This is a proposal to purchase and operate a hybrid quadrupole-time of flight mass spectrometer in the Biomolecular Mass Spectrometry Laboratory of the University of Louisville (UofL). No such high resolution, accurate mass spectrometry for is available now at the UofL or in the region. The Specific Aims of this Proposal are as follows: provision of (1) High resolution, accurate tandem mass spectrometry capabilities for qualitative identification of biologically relevant molecules. (2) Enabling of high sensitivity structural analysis of (2) modified DNA and RNA, (b) post-translational modifications of proteins and (c) non-covalent protein-protein or protein-nucleic acid interactions as biological control mechanisms or markers of xenobiotic toxicity. (3) Enabling of development of quantitative techniques for study of biological control mechanisms at the molecular level. (4) Implementation of an education program for research development and scientific career development. Four Major Users Research Focus Groups have been identified, including 16 Major Users funded by 8 different Institutes of the NIH. The specific applications are quite varied, but are focused in one of four areas in which hybrid Q-TOF technology has been successfully integrated: (1) Cell-cell communications: Signaling and Post- translational modifications, (2) Oxidative processes in pathology and toxicology, (3) Functional Genomics and Proteomics and (4) Molecular Endocrinology. These research scientists will benefit immediately from availability of the Q-TOF for the projects already deemed scientifically important by NIH review, and will have flexible options for user access. An educational outreach strategy will recruit other NIH funded users to incorporate this technology. The University of Louisville makes a strong commitment in support of this application, with an overall value of twice the funds requested. A detailed mechanism is described for efficient management and use of the instrument. The University also commits to paying for the costs of setup, installation, maintenance and support personnel. The commitment of the University is made for a period of five years. The Principal Investigator will rely on advice from a Technical Advisory Group and an Internal Advisory Committee, the composition of which is specified in the proposal. This project will allow these investigators and others to break through a technical roadblock to have their requirements met by in-house analytical capability.

这是一个建议，购买和操作的混合四极杆飞行时间质谱仪在路易斯维尔大学（UofL）的生物分子质谱实验室。目前在UofL或该地区还没有这样高分辨率、准确的质谱仪。本提案的具体目的如下：提供（1）用于生物相关分子定性鉴定的高分辨率、准确的串联质谱能力。(2)能够对（2）修饰的DNA和RNA、（B）蛋白质的翻译后修饰和（c）非共价蛋白质-蛋白质或蛋白质-核酸相互作用进行高灵敏度结构分析，作为生物控制机制或异生质毒性的标记。(3)发展定量技术，在分子水平上研究生物防治机制。(4)实施研究发展和科学职业发展教育方案。已确定了四个主要用户研究焦点组，包括由NIH 8个不同研究所资助的16个主要用户。具体的应用是多种多样的，但是集中在混合Q-TOF技术已经成功整合的四个领域之一：（1）细胞-细胞通信：信号传导和翻译后修饰，（2）病理学和毒理学中的氧化过程，（3）功能基因组学和蛋白质组学以及（4）分子内分泌学。这些研究科学家将立即受益于Q-TOF的可用性，这些项目已经被NIH审查认为具有科学重要性，并且将为用户访问提供灵活的选择。一项教育推广战略将招募其他NIH资助的用户采用这项技术。路易斯维尔大学做出了坚定的承诺，支持这一申请，总价值是所需资金的两倍。一个详细的机制，描述了有效的管理和使用的仪器。大学还承诺支付设置，安装，维护和支持人员的费用。大学的承诺是为期五年。主要调查员将依靠技术咨询小组和内部咨询委员会的咨询意见，提案中具体说明了这两个小组和委员会的组成。该项目将使这些调查人员和其他人员能够突破技术障碍，通过内部分析能力满足他们的要求。