CORE--LABORATORY

核心--实验室

• 批准号: 6564089
• 负责人: JEREMIAH J MORRISSEY
• 金额: $ 18.65万
• 依托单位: BARNES-JEWISH HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-01-01 至 2002-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6564089
• 关键词: actins; animal breeding; animal colony; biomedical facility; gene expression; gene targeting; genetic promoter element; genetically modified animals; histology; in situ hybridization; kidney function; laboratory mouse; laboratory rat; macrophage; molecular biology; monocyte; mutant; reporter genes; tissue /cell culture; urinary tract obstruction

DESCRIPTION (Adapted from the application) This core will function as the principal laboratory core for the program project. Components of the core include 1) an animal and histology core, and 2) an animal and molecular biology core. The animal and histology part of the core will maintain breeding colonies of wild type and mutant mice, perform UUO procedures in rats and mice, and perform histology, interstitial volume measurements, detection of monocytes and macrophages, in situ hybridization, assays for apoptosis, and measurements of renal function. The animal and molecular biology portion of the core will be responsible for cell culture, generation of promoter-reporter and gene targeting constructs, and production of transgenic and knockout mice.

描述（改编自应用程序） 该核心将作为该计划的主要实验室核心 项目核心的组分包括1）动物和组织学核心，以及2） 动物和分子生物学核心。核心的动物和组织学部分 将维持野生型和突变小鼠的繁殖群体，进行UUO 在大鼠和小鼠中的程序，并进行组织学，间质体积 测量，单核细胞和巨噬细胞的检测，原位杂交， 细胞凋亡的测定和肾功能的测量。动物和 核心的分子生物学部分将负责细胞培养， 启动子-报告子和基因靶向构建体的产生，以及 转基因小鼠和基因敲除小鼠。