课题基金 / 基金详情

CORE--LABORATORY

CORE--LABORATORY
核心--实验室
批准号：
6564089
负责人：
JEREMIAH J MORRISSEY
金额：
$18.65万
依托单位：
BARNES-JEWISH HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2002
资助国家：
美国
项目状态：
已结题
起止时间：
2002-01-01 至 2002-12-31
来源：
https://reporter.nih.gov/project-details/6564089
关键词：
actins animal breeding animal colony biomedical facility gene expression gene targeting genetic promoter element genetically modified animals histology in situ hybridization kidney function laboratory mouse laboratory rat macrophage molecular biology monocyte mutant reporter genes tissue /cell culture urinary tract obstruction

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英文摘要
DESCRIPTION (Adapted from the application) This core will function as the principal laboratory core for the program project. Components of the core include 1) an animal and histology core, and 2) an animal and molecular biology core. The animal and histology part of the core will maintain breeding colonies of wild type and mutant mice, perform UUO procedures in rats and mice, and perform histology, interstitial volume measurements, detection of monocytes and macrophages, in situ hybridization, assays for apoptosis, and measurements of renal function. The animal and molecular biology portion of the core will be responsible for cell culture, generation of promoter-reporter and gene targeting constructs, and production of transgenic and knockout mice.
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