Liver Cell Membrane Proteins--Expression and Function

描述(由申请人提供)： 这是一个计划项目，以继续调查功能和 贩卖特定的肝细胞！表膜蛋白。四个项目 涉及代表10个学术部门的12名教职员工调查人员 建议。第一个项目将描述特定的细胞成分 调节囊泡的运输和分类。特异型内质体囊泡 内吞途径的不同阶段以及单纯疱疹病毒囊泡的外吐 将会被检查。我国新型荧光显微镜的最新发展 技术应该会极大地促进这些研究。第二个项目有 描述了缝隙连接蛋白32的基因组调节因子，最近证明 连接蛋白和紧密连接蛋白之间的相互作用。与以下内容交互 关于连接蛋白贩运的其他三个项目仍在继续。第三 该项目的重点是分离和鉴定贩运突变株 在内吞途径中。Trfi突变体是最初的贩卖突变体 那是我们隔离的。补充这种突变的基因已经被克隆并 编码一个新的酪蛋白激酶II催化亚基。第二个突变细胞系 已被隔离，并提出了用于隔离其他 台词。Trfl的贩运缺陷一直是 与其他项目的合作研究。第四个项目是新的，是 肝细胞膜反胆固醇转运的研究进展 运输。小窝蛋白和磷脂酰胆碱转运蛋白在脑内的作用 我们将对这一过程进行审查。此项目已添加到计划中，原因是 与现有三个项目一起发展的协作。本计划 还提供两个核心设施。行政和支持性服务 CORE提供必要的秘书、簿记和通用设备 功能。分子细胞学核心提供细胞化学和形态 服务。所有这些项目都涉及相互关联的领域 肝细胞膜生物学与病理生物学。专家小组的专业知识 调查人员是相辅相成的，能够在 计划框架促进思想、方法和 资源。

DESCRIPTION (provided by applicant): This is a Program Project to continue investigation of the function and trafficking of specific liver cell! surface membrane proteins. Four projects involving 12 faculty investigators representing ten academic departments are proposed. The first Project will characterize specific cellular components mediating vesicle trafficking and sorting. Endosomal vesicles at specific stages of the endocytic pathway as well as exocytosing Herpes simplex vesicles will be examined. Our recent development of novel fluorescence microscopy technologies should greatly facilitate these studies. The second Project has characterized genomic regulators of connexin 32, and has recently demonstrated interactions between connexins and tight junction proteins. Interactions with the three other Projects regarding connexin trafficking continue. The third Project is focused on the isolation and characterization of trafficking mutants in the endocytic pathway. The Trfi mutant is the original trafficking mutant that we isolated. The gene that complements this mutation has been cloned and encodes a novel Casein Kinase II catalytic subunit. A second mutant cell line has been isolated, and protocols are proposed for isolation of additional lines. The trafficking defects of Trfl have been important tools for collaborative studies with the other Projects. The fourth Project is new and is focused on hepatocellular membrane trafficking in reverse cholesterol transport. Roles for caveolin and for phosphatidylcholine transfer protein in this process will be examined. This Project was added to the Program because of collaborations that developed with the existing three Projects. This Program also provides two Core facilities. The Administrative and Supporting Services Core provides the necessary secretarial, bookkeeping, and common equipment functions. The Molecular Cytology Core provides cytochemical and morphologic services. All of these Projects are concerned with interrelated areas of hepatocyte membrane biology and pathobiology. The expertise of the investigators is complementary, and the ability to work and interact within the framework of a Program promotes the sharing of ideas, methodology, and resources.