DVLP WWW BASED NEURAL NETWORK ANALYZER FOR GXM DATABASE: CRYPTOCOCCUS, AIDS

用于 GXM 数据库的基于 DVLP WWW 的神经网络分析仪：隐球菌、艾滋病

• 批准号: 6653541
• 负责人: FARAMARZ VALAFAR
• 金额: $ 28.8万
• 依托单位: UNIVERSITY OF GEORGIA
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2003-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6653541
• 关键词: biological products; biomedical resource; carbohydrates; spectrometry; technology /technique

Fourier transform-infrared (FT-IR) spectroscopy is being developed as a stand-alone method for glycosyl composition analysis and as an adjunct to capillary electrophoretic (CE) separation of the monosaccharide components of glycoconjugates. Early results indicated that FT-IR spectroscopy can be used to differentiate among a wide spectrum of monosaccharide isomers, and that buffers typically used for CE separations do not interfere with IR detection. More recently, a new experimental method has been developed for FT-IR quantitative analysis with the use of FT-IR microscopy. Film spectra of sample solutions deposited and dried on a ZnSe plate were collected with an FT-IR microscope and a high-sensitivity mercury cadmium telluride detector. A fairly extensive library of monosaccharide IR spectra has been collected at high resolution under different conditions. The library is being extended to include di- and higher oligosaccharides. Recent work has focused on the pote ntial of performing glycosyl composition analysis by FT-IR spectroscopy of per-N,O-acetylated derivatives of the monosaccharide methylglycoside mixtures obtained after acidic methanolysis of mono- and oligosaccharides. The peracetylation step was introduced after methanolysis because the monosaccharide methylglycosides were observed to have poor deposition characteristics. Since succesful application of the method will depend on prior acquisition of training spectra from a wide distribution of known samples, including pure monosaccharides as well as standard mixtures, a large number of these samples were generated from combinations of sugars known to comprise N- and O-linked oligosaccharides of mammalian glycoproteins. Spectra from 145 standards of known composition have been acquired, and the method is now being tested on unknown oligosaccharides and monosaccharide mixtures.

傅里叶变换红外光谱（FT-IR）正在开发中 作为糖基组成分析的独立方法， 辅助毛细管电泳（CE）分离 糖缀合物的单糖组分。 早期结果显示， FT-IR光谱可以用来区分广泛的 单糖异构体谱和通常使用的缓冲液 CE分离不干扰IR检测。 最近， 发展了一种新的FT-IR定量分析方法， 使用FT-IR显微镜进行分析。 样品的薄膜光谱 在ZnSe板上沉积和干燥的溶液用收集器收集。 FT-IR显微镜和高灵敏度碲镉汞 检测器 一个相当广泛的单糖红外光谱库， 在不同条件下以高分辨率收集。 的 库正在扩展到包括二-和更高的寡糖。 最近的工作集中在进行糖基化的潜力上， 通过FT-IR光谱分析全-N，O-乙酰化的 得到的单糖甲基糖苷混合物的衍生物 在单糖和寡糖的酸性甲醇分解之后。 的 在甲醇分解之后引入全乙酰化步骤，因为 观察到单糖甲基糖苷具有差的沉积 特色 由于该方法的成功应用将 依赖于训练光谱的先前获取， 已知样品的分布，包括纯单糖以及 作为标准混合物，产生了大量的这些样品 由已知包含N-和O-连接的糖的组合 哺乳动物糖蛋白的寡糖。 光谱145 已获得已知组成的标准品， 目前正在测试未知的低聚糖和单糖 混合物。