NEUROMUSCULAR DEVELOPMENT AFTER BILABYRINTHECTOMY

胆道切除术后的神经肌肉发育

• 批准号: 6626879
• 负责人: MARY S SHALL
• 金额: $ 21.46万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-09 至 2004-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6626879
• 关键词: anatomy; ferrets; growth /development; labyrinthectomy; muscle contraction; myosins; neuromuscular function; physiology; posture; protein isoforms; psychomotor function; vestibular apparatus; videotape /videodisc

DESCRIPTION:(adapted from applicant's abstract) This proposal will examine whether there is a developmentally sensitive period in which vestibular sensation is necessary for the "normal" anatomical and physiological maturation of postural and extraocular muscles as well as motor behavior. The ferret is an excellent model for these studies because it is born with a very immature sensory system. In order to identify the critical period, both vestibular labyrinths will be removed at postnatal day 10, 15, 28, or 60. Behaviorally, the ferret's motor activity and postural reflexes will be recorded on videotape and quantified as the animals develop for 30, 60, or 90 days. Physiologically, the investigators will measure any changes in muscle contractile characteristics such as contraction speed, force and fatigue. Anatomically the investigators will examine changes in muscle fiber type, fiber diameter, muscle fiber type distribution and changes in the proportions of myosin heavy chain isoforms that result from bilateral vestibular deficits. Their major hypotheses are that anatomical and behavioral changes do occur and that there is a sensitive time period when vestibular receptor activity is necessary for normal neuromuscular and motor development.

描述：（改编自申请人摘要） 这项建议将研究是否有一个发展敏感期 其中前庭感觉对于“正常的”解剖学是必需的， 姿势肌和眼外肌以及运动肌的生理成熟 行为雪貂是这些研究的极好模型，因为它出生在 感觉系统还很不成熟为了确定关键时期， 在出生后第10、15、28或60天将两个前庭神经节移除。 从行为上讲，雪貂的运动活动和姿势反射将 在录像带上记录，并在动物发育30、60或90小时时进行定量 天从生理学上讲，研究人员将测量肌肉的任何变化， 收缩特性，如收缩速度、力和疲劳。 从解剖学上讲，研究人员将检查肌纤维类型、纤维 直径、肌纤维类型分布和比例变化 肌球蛋白重链同种型，由双侧前庭缺陷引起。 他们的主要假设是，解剖和行为的变化确实发生， 当前庭感受器活动被激活时， 正常的神经肌肉和运动发育所必需的。