CRYSTALLOGRAPHIC STUDIES OF PROTEINS SDNA COMPLEXES

这个项目的目标是了解蛋白质是如何结合到 单链核酸。单链核酸结合 蛋白质在关键的细胞过程中发挥作用，如DNA复制， RNA翻译的重组、修复和调控。如果进来的路 哪些蛋白质与单链核酸相互作用 详细了解，则有可能修改或更正 人类蛋白质的功能，如复制蛋白A 规模更大，也更难研究。这一点在 治疗因这些细胞的功能缺陷而引起的疾病 蛋白质。我们已经获得了质量高但晶体小的几种 基因5蛋白与单链寡核苷酸结合的复合体。 我们希望，这些结构的确定将给予我们深刻的认识。 到蛋白质-单链DNA的相互作用，这是任何 另一条路。

The goal of this project is to understand how a protein binds to single-stranded nucleic acids. Single-stranded nucleic acid-binding proteins play roles in key cellular processes such as DNA replication, recombination, repair, and control of RNA translation. If the ways in which proteins interact with single-stranded nucleic acids were understood in detail, then it might be possible to modify or correct the functions of human proteins such as replication protein A that are larger and more difficult to study. This would be important in the treatment of diseases due to deficiencies in the function of these proteins. We have obtained high-quality but small crystals of several complexes of gene 5 protein bound to single-stranded oligonucleotides. We hope that the determination of these structures will give insights into protein-ssDNA interactions that could not be obtained in any other way.