STRUCTURE OF N TERMINAL DOMAIN OF SIGNAL SEQUENCE RECOGNITION PROTEIN

信号序列识别蛋白N端结构域的结构

• 批准号: 6586558
• 负责人: DOUGLAS M FREYMANN
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586558
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

Our current effort is directed towards obtaining larger crystals of the NG domain. Due to their small size, rapid decay, and relatively weak diffraction, further crystallographic characterization using laboratory X-ray sources may remain problematic. We are applying for time at SSRL in order to: 1) evaluate the diffraction limit and lifetime of the crystals, 2) collect sufficient data to sufficient resolution to convincingly determine the space group symmetry, 3) evaluate the feasibility of using cryo-methods for data collection, and 4) if possible, collect a complete data set.

我们目前的努力是为了获得更大的晶体 在NG领域。由于它们体积小，腐烂快， 相对较弱的衍射，进一步的结晶学表征 使用实验室X射线源可能仍然存在问题。我们是 在SSRL申请时间，以便：1)评估衍射 晶体的极限和寿命，2)收集足够的数据来 足够的分辨率来令人信服地确定空间群 对称性，3)评价冷冻法用于数据处理的可行性 收集，以及4)如果可能，收集完整的数据集。