STRUCTURE OF N TERMINAL DOMAIN OF SIGNAL SEQUENCE RECOGNITION PROTEIN

我们目前的努力方向是获得更大的晶体 NG 域。 由于其体积小、腐烂快、 相对较弱的衍射，进一步的晶体学表征 使用实验室 X 射线源可能仍然存在问题。 我们是 在 SSRL 申请时间以便： 1) 评估衍射 晶体的极限和寿命，2）收集足够的数据 足够的分辨率来令人信服地确定空间群 对称性，3) 评估使用冷冻方法获取数据的可行性 收集，4) 如果可能，收集完整的数据集。

Our current effort is directed towards obtaining larger crystals of the NG domain. Due to their small size, rapid decay, and relatively weak diffraction, further crystallographic characterization using laboratory X-ray sources may remain problematic. We are applying for time at SSRL in order to: 1) evaluate the diffraction limit and lifetime of the crystals, 2) collect sufficient data to sufficient resolution to convincingly determine the space group symmetry, 3) evaluate the feasibility of using cryo-methods for data collection, and 4) if possible, collect a complete data set.