CRYSTAL STRUCTURE OF FRAGMENTS OF HEAT SHOCK TRANSCRIPTION FACTOR

我们已经确定了DNA结合结构域的结构， 来自乳酸克鲁维酵母热休克转录因子（HSF）。 考虑到结构的1.05 E分辨率， 可以解决的问题。 首先，我们感兴趣的是 不寻常的螺旋，包含一个脯氨酸（导致扭结）和一个 额外的氨基酸（导致凸起）。 已经获得了晶体 用丙氨酸代替脯氨酸， 决心正在进行中。 其次，我们感兴趣的是， 蛋白质结合DNA。 晶体已经获得了一种复合物， DNA结合域及其同源结合位点。 数据 沿着使用在光束线7 - 1上收集的MAD数据， 1-5来确定复合体的结构

We have determined the structure of the DNA binding domain of the heat shock transcription factor (HSF) from Kluyveromyces lactis. Given the 1.05 E resolution of the structure, there are many detailed questions that can be addressed. First, we are interested in an unusual helix that contains a proline (which causes a kink) and an extra amino acid (which causes a bulge). Crystals have been obtained that have an alanine instead of the proline and the structure determination is underway. Second, we are interested in how the protein binds DNA. Crystals have been obtained of a complex between the DNA binding domain and its cognate binding site. The data collected on beamline 7-1 was used along with MAD data collected on 1-5 to determine the structure of the complex.