HIGH RESOLUTION MN KB FLUORESCENCE

高分辨率 MN KB 荧光

• 批准号: 6586706
• 负责人: Stephen P. Cramer
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586706
• 关键词: biomaterials; biomedical resource; spectrometry; structural biology; technology /technique

With the high resolution fluorescence spectrometers (for Mn and Fe) developed in this lab, we have obtained the basic knowledge of the chemical environments of the Mn and Fe model systems as well as refined the spectrometer. The information revealed from the kb fluorescence dependence on the spin states, oxidation states and ligand field strength can be used for studying more complicated protein systems. We would like to apply this new technique to the Photo System II in order to determine the electronic structures of the intermediate states and to nitrogenase to study the active sites and the interactions between them. We would also like to have a chance to develop a spectrometer for Mo, which will help to solve the puzzle of how the Mo functions during the catalytic process in nitrogenase.

采用高分辨率荧光光谱仪(Mn和