XRAY DATA COLLECTION OF RNA POLYMERASE II & DNA CO CRYSTAL

最近获得了RNA聚合酶II/DNA的共晶 在旋转阳极X射线源上的衍射率为5E。鉴于…… 旋转阳极式5E分辨率的显著提高 在同步加速器源上的X射线到3E的平行研究中 仅有聚合酶，共晶体有望与3E发生衍射波 同步加速器束也是如此。冷冻浸泡程序已经被 研制成功，使从一块晶体中收集数据成为可能。我们现在 建议收集高分辨率X射线衍射数据 聚合酶II/DNA共晶体及其重原子衍生物。 目标是获得高质量的衍射数据，用于确定 RNA聚合酶II/DNA复合体的晶体结构

Co-crystals of RNA polymerase II/DNA have been recently obtained with diffraction to 5 E on rotating-anode X-ray source. In view of the significant improvement from 5 E resolution with rotating-anode X-ray to 3 E at the synchrotron source in a parallel study of the polymerase alone, the co-crystal is expected to diffract to 3 E with synchrotron beam as well. A cryo-soaking procedure has already been developed, making data collection from one crystal feasible. We now propose to collect high resolution X-ray diffraction data on polymerase II/DNA cocrystal as well as its heavy atom derivatives. The goal is to obtain high quality diffraction data for determining the crystal structure of RNA polymerase II/DNA complex.