XRAY DATA COLLECTION OF RNA POLYMERASE II & DNA CO CRYSTAL

最近获得了RNA聚合酶II/DNA的共晶体 在双阳极X射线源上衍射至5E。 鉴于 与采用双阳极5E分辨率相比， X射线到3E在同步辐射源的平行研究 单独使用聚合酶，预计共晶体将与3E结合， 同步加速器光束。 低温浸泡程序已经 开发，使数据收集从一个晶体可行。 我们现在 建议收集高分辨率X射线衍射数据， 聚合酶II/DNA共晶体及其重原子衍生物。 目标是获得高质量的衍射数据， RNA聚合酶II/DNA复合物的晶体结构。

Co-crystals of RNA polymerase II/DNA have been recently obtained with diffraction to 5 E on rotating-anode X-ray source. In view of the significant improvement from 5 E resolution with rotating-anode X-ray to 3 E at the synchrotron source in a parallel study of the polymerase alone, the co-crystal is expected to diffract to 3 E with synchrotron beam as well. A cryo-soaking procedure has already been developed, making data collection from one crystal feasible. We now propose to collect high resolution X-ray diffraction data on polymerase II/DNA cocrystal as well as its heavy atom derivatives. The goal is to obtain high quality diffraction data for determining the crystal structure of RNA polymerase II/DNA complex.