STRUCTURAL STUDIES OF KLENTAQ DNA POLYMERASE & ITS COMPLEXES W/ DNA

KLENTAQ DNA 聚合酶的结构研究

• 批准号: 6586530
• 负责人: SERGEY KOROLEV
• 金额: $ 14.32万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-03-01 至 2003-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6586530
• 关键词: bioimaging /biomedical imaging; biomaterials; biomedical resource; proteins; radiography; structural biology

The DNA polymerase from Thermophyllus aquaticus (Taq) is a thermostable enzyme extensively used to generate DNA fragments by the polymerase chain reaction (PCR). Our goal is to study the structural basis for thermostability of this polymerase. An explanation for thermostability may only be obtained by determining the 3-D structure of the enzyme. The aim of the current investigation is to study crystallographically the KlenTaq DNA polymerase and its complexes with DNA.

来自Thermofyllus aquaticus（TAQ）的DNA聚合酶是A 热稳定酶广泛用于生成DNA片段 聚合酶链反应（PCR）。 我们的目标是研究结构 该聚合酶热稳定性的基础。 一个解释 只能通过确定3-D结构才能获得热稳定性 酶。 当前调查的目的是研究 晶体学上Klentaq DNA聚合酶及其复合物与 脱氧核糖核酸。