STRUCTURAL STUDIES OF KLENTAQ DNA POLYMERASE & ITS COMPLEXES W/ DNA

来自水生热叶菌（Thermophyllus aquaticus）的DNA聚合酶（Taq）是一种 热稳定酶，广泛用于产生DNA片段， 聚合酶链反应（PCR）。 我们的目标是研究 这种聚合酶的热稳定性的基础。 一个解释 热稳定性只能通过确定三维结构来获得 的酶。 本次调查的目的是研究 晶体学上KlenTaq DNA聚合酶及其与 DNA.

The DNA polymerase from Thermophyllus aquaticus (Taq) is a thermostable enzyme extensively used to generate DNA fragments by the polymerase chain reaction (PCR). Our goal is to study the structural basis for thermostability of this polymerase. An explanation for thermostability may only be obtained by determining the 3-D structure of the enzyme. The aim of the current investigation is to study crystallographically the KlenTaq DNA polymerase and its complexes with DNA.