STRUCTURAL STUDIES OF KLENTAQ DNA POLYMERASE & ITS COMPLEXES W/ DNA
STRUCTURAL STUDIES OF KLENTAQ DNA POLYMERASE & ITS COMPLEXES W/ DNA
批准号:
6658497
负责人:
SERGEY KOROLEV
金额:
$14.32万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2002
资助国家:
美国
项目状态:
已结题
起止时间:
2002-03-01 至 2003-02-28
中文摘要
水热菌DNA聚合酶(Taq)是一种
耐热酶被广泛用于产生DNA片段
聚合酶链式反应(PCR)。我们的目标是研究结构
该聚合酶的热稳定性基础。对……的解释
只有通过确定三维结构才能获得热稳定性
酶的活性。目前调查的目的是研究
KlenTaq DNA聚合酶及其与KlenTaq DNA聚合酶的配合物
DNA
英文摘要
The DNA polymerase from Thermophyllus aquaticus (Taq) is a
thermostable enzyme extensively used to generate DNA fragments by the
polymerase chain reaction (PCR). Our goal is to study the structural
basis for thermostability of this polymerase. An explanation for
thermostability may only be obtained by determining the 3-D structure
of the enzyme. The aim of the current investigation is to study
crystallographically the KlenTaq DNA polymerase and its complexes with
DNA.
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资助金额:$14.32万
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:SERGEY KOROLEV
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依托单位:--
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