GENETICS OF REPRODUCTION NEUROENDOCRINOLOGY

生殖神经内分泌遗传学

• 批准号: 6590019
• 负责人: William Francis Crowley
• 金额: $ 24.33万
• 依托单位: MASSACHUSETTS GENERAL HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-04-01 至 2003-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6590019
• 关键词: clinical research; cooperative study; family genetics; gene mutation; genetic regulation; genotype; high throughput technology; human subject; hypogonadism; linkage mapping; male reproductive system; molecular biology information system; neuroendocrine system; phenotype; reproductive development

Understanding the genetic and molecular determinants of the neuroendocrine control of reproduction has been an important but elusive goal. Idiopathic hypogonadotropic hypogonadism (IHH) is a human disorder in which selective failure of the neuroendocrine components of sexual maturation occurs in the face of low levels of gonadotropins without any demonstrable anatomic cause. The considerable genetic and phenotypic heterogeneity of this condition suggests it is a rich source of unique information, not only about the neuroendocrine control of reproduction, but also about the development of several other organs. Thus, patients with IHH can be viewed as 'experiments of nature/ in which a series of mutations in genes both specific for the human and key to reproduction can be discovered. Utilizing clinical investigation and phenotyping, biochemical profiling, classical genetic studies, and new genomic tools, this proposal plans to address this issue using several unique resources. A broad spectrum of carefully profiled patients with these disorders has been assembled by the PI over the past 25 years and an evolving database of their phenotypic and genotypic features as well as a growing repository of their serum and DNA samples has been established. In our 1st aim, the 3 genes already known to cause this condition in a minority of IHH cases (KAL, DAX, and GnRH/rec) will be screened in this population. Subsequently, using iterative phenotyping, their full biologic spectrum will be defined in the 2nd aim. High throughput screening technologies will then be used to examine a wide spectrum of new candidate genes in this population and their phenotypes similarly identified in the 3rd aim. Finally, where appropriate, linkage analysis and genome mismatch scanning (GMS) will be employed in IHH families with known autosomal recessive inheritance. Together, this combination of resources and approaches should permit a unique opportunity to gain biologic insights into the genetic control of both GnRH and gonadotropin secretion in the human.

了解神经内分泌控制生殖的遗传和分子决定因素一直是一个重要但难以实现的目标。特发性促性腺激素减退症(IHH)是一种人类疾病，在没有任何解剖学原因的情况下，性成熟的神经内分泌成分在低促性腺激素水平的情况下发生选择性失效。这种疾病相当大的遗传和表型异质性表明它是一个丰富的独特信息来源，不仅关于生殖的神经内分泌控制，而且关于其他几个器官的发育。因此，IHH患者可以被视为“自然界的实验”，在这个实验中，可以发现人类特有的一系列基因突变，也可以发现繁殖的关键基因。利用临床调查和表型分析、生化图谱、经典遗传学研究和新的基因组学工具，这项提案计划利用几个独特的资源来解决这个问题。在过去的25年里，PI收集了一批仔细描述这些疾病的广泛患者，并建立了他们的表型和基因特征的不断演变的数据库以及他们的血清和DNA样本库。在我们的第一个目标中，已知在少数IHH病例(KAL、DAX和GnRH/rec)中导致这种情况的3个基因将在该人群中进行筛查。随后，使用迭代表型，它们的全部生物学谱将在第二个目标中定义。然后将使用高通量筛选技术来检查该人群中的一系列新的候选基因及其在第三个目标中类似地确定的表型。最后，在适当的情况下，将在已知常染色体隐性遗传的IHH家系中进行连锁分析和基因组错配扫描(GMS)。总之，这种资源和方法的结合应该允许一个独特的机会，以获得对人类促性腺激素释放激素和促性腺激素分泌的基因控制的生物洞察力。