CORE--NUDE/SCID MOUSE FACILITY

核心——NUDE/SCID 小鼠设施

• 批准号: 6563716
• 负责人: ESTHER F HAYS
• 金额: $ 16.72万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-12-01 至 2002-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6563716
• 关键词: SCID mouse; animal breeding; animal colony; athymic mouse; biomedical facility; disease /disorder model; leukemia; melanoma; multiple myeloma

Description: Immunodeficient mice are being used as a means to grow both normal and transformed cells and/or tissues in vivo. Athymic mice (nu/nu, Balb background) and severe combined immunodeficient (SCID, C.B.-17 scid/scid) mice are being utilized. The resource provides mice for cancer-related studies, housing and maintenance of animals, and resource personnel to facilitate in vivo experiments. Biosafety Level (BSL) 2 and 3 facilities are available within the resource. The shared resource is located in two separate animal facilities, both under the jurisdiction of the Campus Veterinarian and accredited by the American Association for the Accreditation of Laboratory Animal Care (AAALAC). The second floor of Warren Hall contains three rooms used by the shared resource. A 600 sq. ft. room is used for housing colonies of nudes and SCIDs, for surgical procedures to implant human fetal tissues into SCID mice, and for housing experimental mice. The other two rooms of this facility house experimental animals. One room of 300 sq. ft. is used for BSL 2 studies and can house 175 cages of SCID mice. The facility is used exclusively for cancer-related research. Current studies include oncogenesis, metastasis, and gene transfer with replication-defective vectors. The BSL 3 Facility is 400 sq. ft. and capable of housing 150 mouse cages. It is used to house SCIDhuman mice infected with human retroviruses. A portion of the studies in this facility are cancer related, the rest are AIDS related. Current Center-supported studies include in vivo models of HTLV-induced leukemogenesis. The Core SCID breeding colony is housed in the Center for Health Sciences. Access to the colony is limited to individuals trained in animal husbandry. At present, 30 nu/nu breeding sets are maintained with production of 30-40 pups per month. The SCID facility maintains 75 breeding pairs and produces 250 SCID pups per month. Weaned SCIDS from the core breeding facility are transferred to the Warren Hall facility for use in experiments. Animals in both facilities are maintained under microisolation. As an added precaution, SCID and breeding nude mice are housed in laminar flow racks.

描述：免疫缺陷小鼠被用作生长这两种动物的一种手段 体内正常和转化的细胞和/或组织。无瘤小鼠(Nu/Nu， BALB背景)和严重联合免疫缺陷(SCID，C.B.-17 SCID/SCID) 老鼠正在被利用。该资源为癌症相关的小鼠提供了 动物的研究、住房和养护，以及资源人员 促进活体实验。生物安全等级(BSL)2级和3级设施 在资源中可用。 共享资源位于两个独立的动物设施中，均位于 校园兽医的管辖权和美国认可的 实验动物护理认可协会(AAALAC)。这个 沃伦厅二楼有三个共享资源使用的房间。 600平方英尺英国《金融时报》房间用于容纳裸体和SCID群体，用于 将人类胎儿组织植入SCID小鼠的外科手术，以及 安置实验小鼠。这间设备房的另外两个房间 实验动物。一间300平米的房间。英国《金融时报》用于BSL 2研究和 可容纳175只SCID小鼠的笼子。该设施专门用于 与癌症相关的研究。目前的研究包括肿瘤发生、转移和 利用复制缺陷载体进行基因转移。BSL 3设施是400 平方。英国《金融时报》能够容纳150个老鼠笼。它是用来容纳SCIDHuman的 感染人类逆转录病毒的小鼠。 这个机构的部分研究与癌症有关，其余的是 与艾滋病有关。目前由中心支持的研究包括体内模型 HTLV诱导的白血病发生。 核心SCID繁育群体被安置在健康科学中心。 进入殖民地的机会仅限于受过畜牧业培训的个人。 目前，保持30个NU/NU育种单位，生产30-40个 每个月生一只幼崽。SCID设施维护75对繁育对，并生产 每月250只SCID幼崽。从核心育种设施断奶的SCID是 转移到沃伦霍尔实验室用于实验。中的动物 这两个设施都是在微隔离状态下维护的。作为额外的预防措施， SCID和繁殖的裸鼠被安置在层流货架上。