TESTICULAR NERVOUS SYSTEM OF PRIMATES

灵长类动物的睾丸神经系统

• 批准号: 6592288
• 负责人: HENRYK F URBANSKI
• 金额: $ 11.11万
• 依托单位: OREGON HEALTH & SCIENCE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592288
• 关键词: Mammalia; endocrine gland /system; growth /development; nervous system; reproductive system

Intrinsic neuron-like cells expressing the catecholamine-biosynthetic enzyme tyrosine hydroxylase (TH) have recently been identified in the testis of the prepubertal rhesus monkey. The aims of this study were to characterize the neuron-like nature of these cells, and to examine the distribution and frequency of testicular neuronal elements during postnatal development, puberty, adulthood and old age. Using immunohistochemistry, nerve fibers and cell bodies immunoreactive for the neuronal markers neurofilament 200 (NF-200) and synaptosomal associated protein of 25 kDa (SNAP 25), TH and neuropeptide Y, were identified in perivascular locations, intermingled with interstitial Leydig cells and close to the wall of seminiferous tubules. Moreover, marked age-related differences in the numbers of these neuronal elements became apparent when the NF-200 immunoreactive neuronal elements were quantified. Thus, intrinsic neuron-like cell bodies were found only in the testes fro m immatu re animals (<3 years of age). Conversely, nerve fibers were observed at all ages, although they became more prominent after the pubertal increase in LH and testosterone levels. Interestingly, mast cells, which are known to contain potent regulatory substances, were found in close anatomical proximity to nerve fibers. The number of these cells, positively identified with an antibody to tryptase, increased significantly after puberty following the same developmental pattern as the nerve fibers. These results confirm that the testicular nervous system of the monkey comprises two components, intrinsic nerve cells and extrinsic fibers, both of which are catecholaminergic and peptidergic in nature. Furthermore, both components are highly plastic during development, suggesting a possible participation in the onset of puberty. The intratesticular locations of neuron-like cells and fibers suggest that catecholamines and neuropeptides may have multiple sites of actions, and may affect Leydig cel ls, cells of the tubular wall, and vascular cells directly and/or indirectly via intermediation of mast cells. FUNDING Nonfederal Institutional funds PUBLICATIONS Lue YH, Sinhahikim AP, Machida Y, Mayerhofer A, Urbanski HF, Wang C, Swedloff RS. Accelerated germ cell apoptosis in aging rhesus monkeys. In Endocrine Society Program & Abstracts 80th Annual Meeting (held in New Orleans, LA, June 24-27, 1998), p 381, 1998 (abstract). Frunigieri M, Urbanski HF, Calandra R, Mayerhofer A. Elmentos neuronales testiculares en el mono. Medicina Buenos Aires 58:438, 1998 (abstract).

表达的固有神经元样细胞 儿茶酚胺生物合成酶酪氨酸羟化酶（Th）具有 最近在青春期前恒河神的睾丸中发现了 猴。 这项研究的目的是表征类似神经元的 这些细胞的性质，并检查分布和频率 青春期产后发育过程中睾丸神经元元素的 成年和老年。 使用免疫组织化学，神经纤维和 细胞体对神经元标记神经丝200的免疫反应性200 （NF-200）和突触小体相关蛋白25 kDa（SNAP 25），TH 和神经肽Y在血管周地点鉴定出来， 与间质的Leydig细胞混合，靠近 精神小管。 此外，与年龄相关的明显差异 当NF-200时，这些神经元元素的数量变得显而易见 定量免疫反应性神经元元素。 因此，固有 神经元样细胞体仅在睾丸中发现 动物（<3岁）。 相反，在 所有年龄段，尽管他们在青春期后变得更加突出 LH和睾丸激素水平的增加。 有趣的是，肥大细胞， 已知在 关闭与神经纤维的解剖学接近。 这些数量 细胞，用胰蛋白酶抗体阳性鉴定，增加了 遵循相同的发育模式后的青春期后有重要的 作为神经纤维。 这些结果证实了睾丸 猴子的神经系统包括两个组成部分，固有神经 细胞和外部纤维，它们均为儿茶酚胺能和 本质上是肽吉尼亚。 此外，两个组件都高度 开发过程中的塑料，表明可能参与 青春期的发作。 神经元样细胞的肠内部位 纤维表明儿茶酚胺和神经肽可能具有 多个动作部位，可能会影响leydig cel ls， 管状壁和血管细胞直接和/或间接通过 肥大细胞的中介。 资助非联邦机构资金 出版物Lue YH，Sinhahikim AP，Machida Y，Mayerhofer A，Urbanski HF，​​Wang C，Swedloff RS。 衰老的生殖细胞凋亡加速 恒河猴。 在内分泌协会计划与摘要第80届年度 会议（1998年6月24日至27日在洛杉矶新奥尔良举行），第381页，1998年 （抽象的）。 Frunigieri M，Urbanski HF，Calandra R，MayerhoferA。 Elmentos Neuronales睾丸El Mono。 Medicina Buenos Aires 58：438，1998（摘要）。