HEALTH IMPROVEMENT & COST REDUCTION IN ANIMAL FACILITIES

健康改善

• 批准号: 6487045
• 负责人: ABIGAIL L SMITH
• 金额: $ 40.72万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6487045
• 关键词: Helicobacter; Mus musculus; Pasteurella; Pneumocystis carinii; SCID mouse; Staphylococcus aureus; air filtration; air sampling /monitoring; airborne allergen; ammonia; animal care; animal colony; clinical research; communicable disease control; cost effectiveness; enzyme linked immunosorbent assay; epizootiology; germ free condition; human subject; immediate hypersensitivity; nonbiomedical equipment; occupational disease /disorder; occupational health /safety; polymerase chain reaction; tissue /cell culture; work site

DESCRIPTION (Provided by applicant): The overall goal of the proposed studies is to establish environmental conditions that optimize the health of mice used in biomedical research, provide a safe and comfortable workplace, and minimize the costs associated with doing animal-based research. The methods by which the applicants will approach this goal include: (1) determining housing conditions that minimize pathogen transfer, (2) evaluating effects of culling on reproductive success, (3) identifying husbandry and hygiene practices that reduce caretaker exposure to mouse allergen, and (4) instituting workplace activities that may improve the health of animal caretakers. The investigators will first study the capacity of ventilated caging maintained at "neutral" airflow and negatively ventilated changing tables to reduce worker exposure to allergen and to reduce or eliminate transfer of Pasteurella pneumotropica, Helicobacter spp. and Pneumocystis carinii from infected mice to pathogen-free immunocompromised mice. The applicants will determine reproductive success of mice whose litters are culled or not and housed under different ventilation conditions. The efficacy of water, dilute tannic acid, and dilute sodium hypochlorite used to reduce breathing zone allergen levels during bedding changes will be tested. Skin and clothing of caretakers will be sampled for Staphylococcus aureus upon entry into mouse rooms through air or wet showers and for allergen upon exiting the facility through air or wet showers. In an effort to reduce employee workplace injuries, a functional capacity checklist will be developed and will be used to design an employee exercise program. Lastly, a pre-employment Functional Capacity Assessment tool to help slot new hires in positions for which they are physically capable will be used.

描述(由申请人提供)：拟议研究的总体目标 是建立环境条件，以优化使用的小鼠的健康 在生物医学研究方面，提供安全和舒适的工作场所，并尽量减少 与进行动物研究相关的成本。所采用的方法 申请者将接近这一目标包括：(1)确定住房 最大限度减少病原体传播的条件，(2)评估扑杀的效果 关于繁殖成功，(3)确定符合以下条件的畜牧和卫生做法 减少看门人接触老鼠过敏原，以及(4)建立工作场所 可能改善动物饲养员健康的活动。这个 调查人员将首先研究通风笼子保持在 “中性”气流和负向通风更换工作台以减少工人 暴露于过敏原并减少或消除巴氏杆菌的转移 嗜气性肺炎，幽门螺杆菌。和感染小鼠的卡氏肺孢子虫 到无病原体免疫受损的小鼠。申请者将决定 被扑杀或未被扑杀小鼠的繁殖成功 不同的通风条件。水的功效，稀单宁酸， 以及用于降低呼吸区过敏原水平的稀次氯酸钠 在床上用品期间，将测试变化。护理员的皮肤和衣服将 经空气进入鼠房时，须抽检金黄色葡萄球菌 或湿淋浴，以及通过空气或潮湿离开设施时的过敏原 淋浴。为了努力减少员工的工伤事故，一个职能部门 将制定能力核对表，并将其用于设计员工 锻炼计划。最后，职前功能能力评估 一种工具，帮助新员工进入他们身体上有能力胜任的职位 将会被使用。